HitPredict

Interactor 1: MCF2 (P10911)
Proto-oncogene DBL (Proto-oncogene MCF-2) [Cleaved into: MCF2-transforming protein; DBL-transforming protein]
Gene: MCF2

Interactor 2: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.866

Property	Details
Experiments	32203420, 10854437, unassigned2213
Identification Methods	affinity chromatography technology, pull down, fluorescent resonance energy transfer, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	PH~Ras, Ras~RhoGEF
Common GO terms	GO:0005829 - cytosol GO:0005856 - cytoskeleton
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 925 Hs			1 193
+	Hs
		500934 MCF2_HUMAN (1-925): 0: 623: 100.00		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		500931 MCF2_HUMAN (1-925): 0: 623: 100.00		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33
+	Hs
		166479 MCF2L_HUMAN (1-911): 0: 596: 45.55		RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00
+	Hs
		166482 MCF2L_HUMAN (1-911): 0: 596: 45.55		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		166478 MCF2L_HUMAN (1-911): 0: 596: 45.55		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33
+	Hs
		894327 PKH4B_HUMAN (1-892): 8.28e-110: 373: 19.12		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33
+	Hs
		900546 PKHG4_HUMAN (2-896): 2.19e-105: 355: 20.04		RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00
+	Hs
		925330 PKHG4_HUMAN (2-896): 2.19e-105: 355: 20.04		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		894092 PKHG4_HUMAN (2-896): 2.19e-105: 355: 20.04		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33
+	Hs
		545418 PKHG4_HUMAN (2-896): 2.19e-105: 355: 20.04		RAC2_HUMAN (1-193): 3.9e-76: 228: 53.61
+	Hs
		892408 PKHG4_HUMAN (2-896): 2.19e-105: 355: 20.04		RAC3_HUMAN (1-179): 4.85e-74: 223: 57.54
+	Dm
		115195 M9PEF7_DROME (56-872): 2.15e-85: 303: 19.82		RAC1_DROME (1-193): 2.11e-77: 231: 54.12
+	Hs
		925461 ARH40_HUMAN (61-921): 3.91e-80: 286: 16.83		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		925527 SPT13_HUMAN (472-842): 2.24e-83: 283: 23.32		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		900947 ARHG4_HUMAN (468-835): 1.73e-81: 279: 23.70		RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00
+	Hs
		894243 ARH40_HUMAN (61-921): 3.91e-80: 286: 16.83		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33
+	Hs
		545519 ARH40_HUMAN (61-921): 3.91e-80: 286: 16.83		RAC2_HUMAN (1-193): 3.9e-76: 228: 53.61
+	Hs
		894323 SPT13_HUMAN (472-842): 2.24e-83: 283: 23.32		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33
+	Hs
		892502 ARH40_HUMAN (61-921): 3.91e-80: 286: 16.83		RAC3_HUMAN (1-179): 4.85e-74: 223: 57.54
+	Hs
		894480 ARHG4_HUMAN (468-835): 1.73e-81: 279: 23.70		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33