HitPredict

Interactor 1: ACTN1 (P12814)
Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform) (F-actin cross-linking protein) (Non-muscle alpha-actinin-1)
Gene: ACTN1

Interactor 2: PDLI4 (P50479)
PDZ and LIM domain protein 4 (LIM protein RIL) (Reversion-induced LIM protein)
Gene: PDLIM4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.44	0.60	0.515

Property	Details
Experiments	unassigned1304
Identification Methods	two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0001725 - stress fiber GO:0005737 - cytoplasm GO:0030018 - Z disc GO:0030036 - actin cytoskeleton organization GO:0042803 - protein homodimerization activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 892 Hs			1 330
+	Hs
		117245 ACTN1_HUMAN (1-892): 0: 697: 100.00		PDLI1_HUMAN (2-324): 6.41e-52: 176: 42.25
+	Rn
		1149817 ACTN1_RAT (1-892): 0: 698: 99.33		PDLI2_RAT (1-311): 2.84e-43: 154: 28.45
+	Hs
		521616 ACTN1_HUMAN (1-892): 0: 697: 100.00		SYP2L_HUMAN (5-248): 2.67e-34: 134: 23.29
+	Hs
		117234 ACTN4_HUMAN (11-892): 0: 642: 86.74		PDLI1_HUMAN (2-324): 6.41e-52: 176: 42.25
+	Hs
		117270 ACTN3_HUMAN (18-892): 0: 642: 78.51		PDLI1_HUMAN (2-324): 6.41e-52: 176: 42.25
+	Hs
		117257 ACTN2_HUMAN (10-892): 0: 640: 80.29		PDLI1_HUMAN (2-324): 6.41e-52: 176: 42.25
+	Hs
		725662 ACTN2_HUMAN (10-892): 0: 640: 80.29		PDLI3_HUMAN (1-325): 6.49e-42: 151: 31.87
+	Mm
		1337461 ACTN2_MOUSE (10-892): 0: 643: 80.63		LDB3_MOUSE (82-314): 1.54e-35: 138: 15.81
+	Hs
		993296 ACTN3_HUMAN (18-892): 0: 642: 78.51		SYP2L_HUMAN (5-248): 2.67e-34: 134: 23.29
+	Hs
		725700 ACTN2_HUMAN (10-892): 0: 640: 80.29		SYP2L_HUMAN (5-248): 2.67e-34: 134: 23.29
+	Hs
		521598 ACTN1_HUMAN (1-892): 0: 697: 100.00		MAGI1_HUMAN (6-216): 3.73e-11: 66.3: 14.55
+	Hs
		254383 ACTN1_HUMAN (1-892): 0: 697: 100.00		LDB3_HUMAN (226-307): 3.08e-10: 63.2: 17.86
+	Hs
		521623 ACTN1_HUMAN (1-892): 0: 697: 100.00		PDLI7_HUMAN (216-305): 1.44e-09: 60.9: 17.20
+	Mm
		1186636 ACTN1_MOUSE (1-892): 0: 694: 99.22		PDLI5_MOUSE (238-307): 2.39e-10: 63.5: 20.83
+	Dm
		574823 ACTN_DROME (2-892): 0: 641: 67.39		Q95TZ7_DROME (12-151): 4.23e-15: 77.2: 29.29
+	Hs
		211645 ACTN4_HUMAN (11-892): 0: 642: 86.74		MAGI1_HUMAN (6-216): 3.73e-11: 66.3: 14.55
+	Mm
		1267826 ACTN2_MOUSE (10-892): 0: 643: 80.63		GRIP1_MOUSE (12-117): 2.29e-10: 63.9: 25.44
+	Dm
		54833 ACTN_DROME (2-892): 0: 641: 67.39		ZASP_DROME (222-305): 2.69e-10: 63.6: 20.93
+	Hs
		254385 ACTN2_HUMAN (10-892): 0: 640: 80.29		LDB3_HUMAN (226-307): 3.08e-10: 63.2: 17.86
+	Hs
		211672 ACTN4_HUMAN (11-892): 0: 642: 86.74		PDLI7_HUMAN (216-305): 1.44e-09: 60.9: 17.20