HitPredict

Interactor 1: ACTN1 (P12814)
Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform) (F-actin cross-linking protein) (Non-muscle alpha-actinin-1)
Gene: ACTN1

Interactor 2: NHRF2 (Q15599)
Na(+)/H(+) exchange regulatory cofactor NHE-RF2 (NHERF-2) (NHE3 kinase A regulatory protein E3KARP) (SRY-interacting protein 1) (SIP-1) (Sodium-hydrogen exchanger regulatory factor 2) (Solute carrier family 9 isoform A3 regulatory factor 2) (Tyrosine kinase activator protein 1) (TKA-1)
Gene: NHERF2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	0.60	0.536

Property	Details
Experiments	27173435
Identification Methods	affinity chromatography technology, tandem affinity purification
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005886 - plasma membrane GO:0005925 - focal adhesion GO:0070062 - extracellular exosome
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 892 Hs			1 337
+	Hs
		211585 ACTN4_HUMAN (11-892): 0: 642: 86.74		NHRF2_HUMAN (1-337): 6.02e-79: 246: 100.00
+	Hs
		521598 ACTN1_HUMAN (1-892): 0: 697: 100.00		MAGI1_HUMAN (2-204): 1.44e-16: 83.1: 21.95
+	Hs
		521540 ACTN1_HUMAN (1-892): 0: 697: 100.00		SCRIB_HUMAN (4-185): 3.63e-13: 72.7: 18.37
+	Mm
		49891 A1BN54_MOUSE (1-892): 0: 684: 97.20		DLG4_MOUSE (106-233): 1.85e-14: 76.2: 21.48
+	Hs
		211645 ACTN4_HUMAN (11-892): 0: 642: 86.74		MAGI1_HUMAN (2-204): 1.44e-16: 83.1: 21.95
+	Hs
		725643 ACTN2_HUMAN (10-892): 0: 640: 80.29		DLG4_HUMAN (106-233): 1.85e-14: 76.2: 21.48
+	Mm
		885081 ACTN4_MOUSE (11-892): 0: 639: 86.74		DLG4_MOUSE (106-233): 1.85e-14: 76.2: 21.48
+	Hs
		211579 ACTN4_HUMAN (11-892): 0: 642: 86.74		SCRIB_HUMAN (4-185): 3.63e-13: 72.7: 18.37
+	Hs
		993251 ACTN3_HUMAN (18-892): 0: 642: 78.51		SCRIB_HUMAN (4-185): 3.63e-13: 72.7: 18.37
+	Hs
		725649 ACTN2_HUMAN (10-892): 0: 640: 80.29		DLG1_HUMAN (87-235): 1.55e-13: 73.5: 17.31
+	Hs
		725654 ACTN2_HUMAN (10-892): 0: 640: 80.29		SCRIB_HUMAN (4-185): 3.63e-13: 72.7: 18.37
+	Ce
		105571 Q23158_CAEEL (359-891): 1.67e-13: 77: 13.14		DLG1_CAEEL (4-159): 3.71e-25: 108: 19.11
+	Ce
		105581 Q9XVU8_CAEEL (143-891): 1.5e-13: 77: 13.60		DLG1_CAEEL (4-159): 3.71e-25: 108: 19.11
+	Ce
		86403 Q23158_CAEEL (359-891): 1.67e-13: 77: 13.14		C1P633_CAEEL (14-234): 3e-17: 85: 19.19
+	Ce
		88177 H2L2C8_CAEEL (359-891): 1.92e-13: 76.6: 13.31		D3YT29_CAEEL (14-234): 1.98e-16: 82.3: 19.19
+	Mm
		1143324 SPTB2_MOUSE (196-761): 1.13e-14: 81.2: 12.88		DLG4_MOUSE (106-233): 1.85e-14: 76.2: 21.48