HitPredict

Interactor 1: ACTN1 (P12814)
Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform) (F-actin cross-linking protein) (Non-muscle alpha-actinin-1)
Gene: ACTN1

Interactor 2: MILK2 (Q8IY33)
MICAL-like protein 2 (Junctional Rab13-binding protein) (Molecule interacting with CasL-like 2) (MICAL-L2)
Gene: MICALL2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.75	1.00	0.871

Property	Details
Experiments	25416956, 32296183, unassigned1304
Identification Methods	two hybrid, validated two hybrid, two hybrid prey pooling approach, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	CH~bMERB_dom, CH~CH, CH~EFhand_Ca_insen, CH~Spectrin
Common GO terms	GO:0001725 - stress fiber GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0005911 - cell-cell junction GO:0030036 - actin cytoskeleton organization and 1 others more... GO:0051015 - actin filament binding
Homologous interactions	15

Homologous interactions

Color coding for hit scores

	1 892 Hs			1 904
+	Hs
		211610 ACTN4_HUMAN (11-892): 0: 642: 86.74		MILK2_HUMAN (1-904): 9.07e-111: 363: 100.00
+	Hs
		993273 ACTN3_HUMAN (18-892): 0: 642: 78.51		MILK2_HUMAN (1-904): 9.07e-111: 363: 100.00
+	Hs
		521530 ACTN1_HUMAN (1-892): 0: 697: 100.00		ACTN3_HUMAN (2-219): 8.29e-70: 251: 30.28
+	Hs
		70398 ACTN1_HUMAN (1-892): 0: 697: 100.00		B2R8Y4_HUMAN (2-219): 2.04e-69: 249: 30.28
+	Hs
		521485 ACTN1_HUMAN (1-892): 0: 697: 100.00		ACTN2_HUMAN (2-219): 3.17e-68: 246: 28.44
+	Hs
		521452 ACTN1_HUMAN (1-892): 0: 697: 100.00		ACTN1_HUMAN (2-218): 1.94e-67: 244: 28.57
+	Hs
		211478 ACTN1_HUMAN (1-892): 0: 697: 100.00		ACTN4_HUMAN (2-219): 5.5e-67: 243: 27.98
+	Rn
		1167662 ACTN1_RAT (1-892): 0: 698: 99.33		Q6XD99_RAT (2-219): 3.33e-61: 229: 30.28
+	Hs
		70394 B2R8Y4_HUMAN (18-892): 0: 646: 78.74		B2R8Y4_HUMAN (2-219): 2.04e-69: 249: 30.28
+	Hs
		993246 ACTN3_HUMAN (18-892): 0: 642: 78.51		ACTN3_HUMAN (2-219): 8.29e-70: 251: 30.28
+	Hs
		70396 B2R8Y4_HUMAN (18-892): 0: 646: 78.74		ACTN4_HUMAN (2-219): 5.5e-67: 243: 27.98
+	Hs
		725648 ACTN3_HUMAN (18-892): 0: 642: 78.51		ACTN2_HUMAN (2-219): 3.17e-68: 246: 28.44
+	Hs
		211430 ACTN4_HUMAN (11-892): 0: 642: 86.74		ACTN4_HUMAN (2-219): 5.5e-67: 243: 27.98
+	Hs
		211571 ACTN3_HUMAN (18-892): 0: 642: 78.51		ACTN4_HUMAN (2-219): 5.5e-67: 243: 27.98
+	Hs
		993274 ACTN3_HUMAN (18-892): 0: 642: 78.51		MILK1_HUMAN (2-885): 2.52e-61: 225: 29.81