HitPredict

Interactor 1: PHYB (P14713)
Phytochrome B (Protein LONG HYPOCOTYL 3) (Protein OUT OF PHASE 1)
Gene: PHYB

Interactor 2: PIF1 (Q8GZM7)
Transcription factor PIF1 (Basic helix-loop-helix protein 15) (AtbHLH15) (bHLH 15) (Phytochrome-interacting factor 1) (Protein PHYTOCHROME INTERACTING FACTOR 3-LIKE 5) (Transcription factor EN 101) (bHLH transcription factor bHLH015)
Gene: PIF1

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.87	1.00	0.935

Property	Details
Experiments	15448264, 18539749, 15486102, 21928113, 19255368
Identification Methods	pull down, affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	HLH~PAS
Common GO terms	GO:0005634 - nucleus GO:0009409 - response to cold GO:0010029 - regulation of seed germination GO:0010161 - red light signaling pathway
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 1172 At			1 478
+	At
		539664 PHYB_ARATH (19-1172): 0: 1308: 100.00		PIF5_ARATH (17-468): 5.9e-38: 145: 22.29
+	At
		539670 PHYB_ARATH (19-1172): 0: 1308: 100.00		PIL1_ARATH (25-455): 1.19e-36: 141: 21.25
+	At
		539673 PHYB_ARATH (19-1172): 0: 1308: 100.00		PIF4_ARATH (23-438): 1.02e-33: 133: 26.57
+	At
		539676 PHYB_ARATH (19-1172): 0: 1308: 100.00		HFR1_ARATH (250-411): 4.13e-34: 131: 33.92
+	At
		285436 PHYB_ARATH (19-1172): 0: 1308: 100.00		PIF3_ARATH (27-415): 1.21e-31: 129: 21.83
+	At
		539669 PHYB_ARATH (19-1172): 0: 1308: 100.00		PIF6_ARATH (161-423): 1.08e-31: 126: 25.18
+	At
		539661 PHYB_ARATH (19-1172): 0: 1308: 100.00		PIF7_ARATH (179-354): 1.14e-29: 121: 34.44
+	At
		539631 PHYA_ARATH (33-1154): 0: 1131: 51.68		PIF1_ARATH (1-478): 8.72e-90: 283: 100.00
+	At
		285438 PHYD_ARATH (20-1152): 0: 1238: 82.17		PIF3_ARATH (27-415): 1.21e-31: 129: 21.83
+	At
		539627 PHYA_ARATH (33-1154): 0: 1131: 51.68		PIF5_ARATH (17-468): 5.9e-38: 145: 22.29
+	At
		539634 PHYA_ARATH (33-1154): 0: 1131: 51.68		PIF4_ARATH (23-438): 1.02e-33: 133: 26.57
+	At
		539635 PHYA_ARATH (33-1154): 0: 1131: 51.68		HFR1_ARATH (250-411): 4.13e-34: 131: 33.92
+	At
		285435 PHYA_ARATH (33-1154): 0: 1131: 51.68		PIF3_ARATH (27-415): 1.21e-31: 129: 21.83
+	At
		285437 PHYC_ARATH (37-1151): 0: 1086: 50.67		PIF3_ARATH (27-415): 1.21e-31: 129: 21.83