HitPredict

Interactor 1: BRAF (P15056)
Serine/threonine-protein kinase B-raf (EC 2.7.11.1) (Proto-oncogene B-Raf) (p94) (v-Raf murine sarcoma viral oncogene homolog B1)
Gene: BRAF

Interactor 2: 1433T (P27348)
14-3-3 protein theta (14-3-3 protein T-cell) (14-3-3 protein tau) (Protein HS1)
Gene: YWHAQ

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	1.00	0.980

Property	Details
Experiments	17353931, 33961781, 36931259, 28514442, 27034005, 27684187, 31015455, 31980649, 32707033, 18332145, 17979178, unassigned2173, 15324660
Identification Methods	affinity chromatography technology, proximity labelling technology, tandem affinity purification, anti tag coimmunoprecipitation, pull down, anti bait coimmunoprecipitation, coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	14-3-3~C1_1, 14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 766 Hs			1 245
+	Hs
		542849 BRAF_HUMAN (1-766): 0: 727: 100.00		1433E_HUMAN (2-243): 1.31e-144: 406: 63.53
+	Hs
		110869 H7C560_HUMAN (1-763): 0: 721: 99.87		1433E_HUMAN (2-243): 1.31e-144: 406: 63.53
+	Hs
		542857 BRAF_HUMAN (1-766): 0: 727: 100.00		1433Z_HUMAN (1-245): 1.75e-141: 397: 79.59
+	Hs
		110870 H7C560_HUMAN (1-763): 0: 721: 99.87		1433Z_HUMAN (1-245): 1.75e-141: 397: 79.59
+	Hs
		542827 BRAF_HUMAN (1-766): 0: 727: 100.00		1433B_HUMAN (1-242): 5.01e-139: 391: 81.82
+	Gg
		982842 BRAF_CHICK (18-766): 0: 709: 95.08		1433E_CHICK (2-243): 1.31e-144: 406: 63.53
+	Gg
		982840 BRAF_CHICK (18-766): 0: 709: 95.08		1433T_CHICK (1-245): 3.09e-141: 397: 98.78
+	Gg
		982836 BRAF_CHICK (18-766): 0: 709: 95.08		1433Z_CHICK (1-245): 7.94e-141: 396: 79.18
+	Hs
		46556 A0A2R8Y8E0_HUMAN (1-763): 0: 699: 94.89		1433B_HUMAN (1-242): 5.01e-139: 391: 81.82
+	Mm
		655938 BRAF_MOUSE (12-766): 0: 678: 91.94		1433E_MOUSE (2-243): 1.31e-144: 406: 63.53
+	Gg
		982838 BRAF_CHICK (18-766): 0: 709: 95.08		1433B_CHICK (1-242): 6.39e-126: 358: 80.99
+	Hs
		542828 BRAF_HUMAN (1-766): 0: 727: 100.00		1433S_HUMAN (1-236): 9.86e-108: 312: 71.85
+	Hs
		542868 BRAF_HUMAN (1-766): 0: 727: 100.00		1433F_HUMAN (2-242): 7.16e-106: 307: 71.43
+	Hs
		542847 BRAF_HUMAN (1-766): 0: 727: 100.00		1433G_HUMAN (1-242): 5.48e-105: 305: 70.33
+	Gg
		982837 BRAF_CHICK (18-766): 0: 709: 95.08		Q5ZKJ2_CHICK (2-242): 5.28e-106: 308: 71.43
+	Gg
		982833 BRAF_CHICK (18-766): 0: 709: 95.08		1433G_CHICK (1-242): 2.55e-105: 306: 70.33
+	Hs
		46558 A0A2R8Y8E0_HUMAN (1-763): 0: 699: 94.89		1433F_HUMAN (2-242): 7.16e-106: 307: 71.43
+	Hs
		46557 A0A2R8Y8E0_HUMAN (1-763): 0: 699: 94.89		1433G_HUMAN (1-242): 5.48e-105: 305: 70.33
+	Mm
		250867 BRAF_MOUSE (12-766): 0: 678: 91.94		1433S_MOUSE (1-232): 2e-106: 309: 72.65
+	Mm
		655936 BRAF_MOUSE (12-766): 0: 678: 91.94		1433G_MOUSE (1-242): 5.48e-105: 305: 70.33