HitPredict

Interactor 1: ERF3A (P15170)
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (Eukaryotic peptide chain release factor subunit 3a) (eRF3a) (EC 3.6.5.-) (G1 to S phase transition protein 1 homolog)
Gene: GSPT1

Interactor 2: U5S1 (Q15029)
116 kDa U5 small nuclear ribonucleoprotein component (Elongation factor Tu GTP-binding domain-containing protein 2) (SNU114 homolog) (hSNU114) (U5 snRNP-specific protein, 116 kDa) (U5-116 kDa)
Gene: EFTUD2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.789

Property	Details
Experiments	28515276, 35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	EFG_C~GTP_EFTU, EFG_III~GTP_EFTU, EFG_IV~GTP_EFTU, EFTUD2~GTP_EFTU, GTP_EFTU~GTP_EFTU, GTP_EFTU~GTP_EFTU_D2, EFG_III~GTP_EFTU_D2, EFTUD2~GTP_EFTU_D2, GTP_EFTU_D2~GTP_EFTU_D2, GTP_EFTU~GTP_EFTU_D3, GTP_EFTU_D2~GTP_EFTU_D3
Common GO terms	GO:0003723 - RNA binding GO:0003924 - GTPase activity GO:0005525 - GTP binding GO:0005829 - cytosol
Homologous interactions	6

Homologous interactions

Color coding for hit scores

	1 499 Hs			1 972
+	Hs
		932705 EF1A1_HUMAN (70-498): 1.24e-175: 503: 38.29		U5S1_HUMAN (1-972): 0: 1062: 100.00
+	Hs
		1064889 HBS1L_HUMAN (3-496): 1.8e-164: 483: 38.11		U5S1_HUMAN (1-972): 0: 1062: 100.00
+	Hs
		531115 EF1A1_HUMAN (70-498): 1.24e-175: 503: 38.29		EF2_HUMAN (111-957): 0: 915: 38.16
+	Sc
		367837 EF1A_YEAST (70-494): 1.07e-172: 495: 37.27		EF2_YEAST (111-957): 0: 879: 36.04
+	Sc
		402906 ERF3_YEAST (72-494): 7.68e-168: 491: 57.51		EF2_YEAST (111-957): 0: 879: 36.04
+	Zm
		5859 O50018_MAIZE (70-499): 1.75e-172: 495: 38.57		A0A1D6E4M0_MAIZE (120-924): 0: 579: 20.35