HitPredict

Interactor 1: UBC4 (P15731)
Ubiquitin-conjugating enzyme E2 4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme 4) (Ubiquitin carrier protein 4) (Ubiquitin-protein ligase 4)
Gene: UBC4

Interactor 2: UFD4 (P33202)
Ubiquitin fusion degradation protein 4 (UB fusion protein 4) (EC 2.3.2.-) (HECT-type E3 ubiquitin transferase UFD4) (EC 2.3.2.26)
Gene: UFD4

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.85	1.00	0.924

Property	Details
Experiments	11805837, 16554755, 37968396, 10089879, 21076411
Identification Methods	anti tag coimmunoprecipitation, affinity chromatography technology, pull down, tandem affinity purification
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	HECT~UQ_con
Common GO terms	GO:0000209 - protein polyubiquitination GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0006511 - ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 148 Sc			1 1483
+	Hs
		1056647 UB2D4_HUMAN (2-148): 4.02e-83: 242: 79.59		TRIPC_HUMAN (129-1483): 0: 913: 20.98
+	Hs
		849331 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		TRIPC_HUMAN (129-1483): 0: 913: 20.98
+	Hs
		125808 UB2D4_HUMAN (2-148): 4.02e-83: 242: 79.59		WWP2_HUMAN (610-1480): 0: 722: 16.39
+	Hs
		125262 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		WWP2_HUMAN (610-1480): 0: 722: 16.39
+	Hs
		125335 UB2D2_HUMAN (2-148): 3.09e-82: 239: 78.91		WWP2_HUMAN (610-1480): 0: 722: 16.39
+	Hs
		849444 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		WWP1_HUMAN (640-1480): 0: 660: 16.18
+	Hs
		1294888 UB2D4_HUMAN (2-148): 4.02e-83: 242: 79.59		ITCH_HUMAN (827-1480): 0: 614: 17.64
+	Hs
		849411 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		ITCH_HUMAN (827-1480): 0: 614: 17.64
+	Hs
		849416 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		NED4L_HUMAN (822-1482): 0: 597: 16.37
+	Hs
		808052 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		NEDD4_HUMAN (801-1482): 3.61e-180: 575: 17.77
+	Sc
		549815 UBC4_YEAST (1-148): 2.13e-82: 240: 100.00		RSP5_YEAST (774-1480): 1.01e-176: 550: 15.57
+	Sc
		549857 UBC5_YEAST (1-148): 6.96e-82: 239: 92.57		RSP5_YEAST (774-1480): 1.01e-176: 550: 15.57
+	Hs
		849451 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		SMUF2_HUMAN (905-1479): 2.06e-176: 547: 17.30
+	Hs
		849452 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		SMUF1_HUMAN (965-1479): 4.08e-162: 509: 17.15
+	Hs
		849333 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		HERC3_HUMAN (826-1481): 1.62e-149: 485: 19.36
+	Hs
		1357018 UB2D4_HUMAN (2-148): 4.02e-83: 242: 79.59		HECD1_HUMAN (1277-1475): 3.61e-27: 123: 35.44
+	Hs
		849486 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		HECD1_HUMAN (1277-1475): 3.61e-27: 123: 35.44
+	Hs
		849334 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		UBE3C_HUMAN (1185-1478): 1.15e-26: 120: 29.43
+	Hs
		849311 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		UBE3A_HUMAN (1204-1482): 2.39e-21: 103: 29.17
+	Hs
		849353 UB2D1_HUMAN (2-148): 1.97e-82: 240: 78.23		HUWE1_HUMAN (1191-1483): 1.44e-20: 101: 31.51