HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: SOS1 (Q07889)
Son of sevenless homolog 1 (SOS-1)
Gene: SOS1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.969

Property	Details
Experiments	32203420, 8810325, 10206341, 7862111, 10026169, 33961781, 17474147
Identification Methods	affinity chromatography technology, pull down, peptide array, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	PH~SH3_1, RhoGEF~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007165 - signal transduction GO:0140693 - molecular condensate scaffold activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 1333
+	Hs
		209038 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		SOS1_HUMAN (1-1333): 0: 991: 100.00
+	Hs
		557669 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		SOS2_HUMAN (1-1332): 0: 855: 68.03
+	Hs
		992475 GRAP_HUMAN (5-251): 9.35e-27: 107: 20.00		SOS1_HUMAN (1-1333): 0: 991: 100.00
+	Hs
		655332 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		SOS1_HUMAN (1-1333): 0: 991: 100.00
+	Hs
		412150 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		SOS1_HUMAN (1-1333): 0: 991: 100.00
+	Hs
		442816 HCK_HUMAN (2-174): 4.07e-20: 93.7: 17.24		SOS1_HUMAN (1-1333): 0: 991: 100.00
+	Hs
		992477 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		SOS1_HUMAN (1-1333): 0: 991: 100.00
+	Hs
		655333 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		SOS2_HUMAN (1-1332): 0: 855: 68.03
+	Hs
		412151 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		SOS2_HUMAN (1-1332): 0: 855: 68.03
+	Dm
		644374 SRC42_DROME (6-127): 2.31e-18: 88.3: 24.19		SOS_DROME (8-1305): 0: 692: 42.34
+	Hs
		557683 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		RPGF1_HUMAN (571-1032): 8.27e-91: 320: 23.63
+	Hs
		557756 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		RPGF5_HUMAN (522-1018): 7.32e-85: 291: 16.12
+	Hs
		655361 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		RPGF1_HUMAN (571-1032): 8.27e-91: 320: 23.63
+	Hs
		548654 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		VAV_HUMAN (134-691): 1.71e-76: 274: 14.41
+	Hs
		412185 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		RPGF1_HUMAN (571-1032): 8.27e-91: 320: 23.63
+	Hs
		442826 HCK_HUMAN (2-174): 4.07e-20: 93.7: 17.24		RPGF1_HUMAN (571-1032): 8.27e-91: 320: 23.63
+	Hs
		557709 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		RGPS1_HUMAN (774-945): 3.81e-23: 107: 31.43
+	Hs
		548645 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		VAV_HUMAN (134-691): 1.71e-76: 274: 14.41
+	Hs
		411981 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		VAV_HUMAN (134-691): 1.71e-76: 274: 14.41
+	Hs
		224699 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		KALRN_HUMAN (8-625): 2.16e-64: 244: 12.85