HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: LCP2 (Q13094)
Lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of 76 kDa) (SLP-76 tyrosine phosphoprotein) (SLP76)
Gene: LCP2

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	1.00	0.967

Property	Details
Experiments	15388330, 10229072, 22267732, 9846482, unassigned1913, 21725281, 20562827, 15929943
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation, split luciferase complementation, protein complementation assay, coimmunoprecipitation, anti bait coimmunoprecipitation, isothermal titration calorimetry, cosedimentation in solution
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	SH2~SH2, SH2~SH3_1
Common GO terms	GO:0005829 - cytosol GO:0005911 - cell-cell junction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 533
+	Mm
		1141384 NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94		LCP2_MOUSE (1-531): 8.21e-72: 240: 80.98
+	Hs
		209043 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		LCP2_HUMAN (1-533): 1.59e-78: 258: 100.00
+	Hs
		557750 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		BLNK_HUMAN (75-520): 4.29e-35: 139: 24.28
+	Hs
		209129 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		BLNK_HUMAN (75-520): 4.29e-35: 139: 24.28
+	Hs
		203208 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		SYNJ1_HUMAN (10-466): 1.18e-22: 104: 11.71
+	Hs
		655347 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		LCP2_HUMAN (1-533): 1.59e-78: 258: 100.00
+	Hs
		412165 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		LCP2_HUMAN (1-533): 1.59e-78: 258: 100.00
+	Hs
		442821 HCK_HUMAN (2-174): 4.07e-20: 93.7: 17.24		LCP2_HUMAN (1-533): 1.59e-78: 258: 100.00
+	Mm
		763395 FYN_MOUSE (7-173): 7.13e-21: 96: 15.39		LCP2_MOUSE (1-531): 8.21e-72: 240: 80.98
+	Hs
		655407 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		CLNK_HUMAN (182-528): 1.05e-42: 159: 25.00
+	Mm
		763411 FYN_MOUSE (7-173): 7.13e-21: 96: 15.39		CLNK_MOUSE (150-528): 6.78e-37: 143: 28.57
+	Mm
		536209 FGR_MOUSE (6-150): 1.5e-18: 89.1: 16.33		CLNK_MOUSE (150-528): 6.78e-37: 143: 28.57
+	Rn
		192744 SRBS2_RAT (3-162): 4.86e-25: 109: 28.05		SYNJ1_RAT (10-456): 4.75e-21: 99.7: 14.26
+	Hs
		203192 SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26		SYNJ1_HUMAN (10-466): 1.18e-22: 104: 11.71
+	Hs
		655419 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		TENS4_HUMAN (98-512): 8.78e-21: 98.2: 14.12
+	Hs
		203230 SH319_HUMAN (8-254): 6.26e-19: 90.5: 19.84		SYNJ1_HUMAN (10-466): 1.18e-22: 104: 11.71
+	Hs
		203228 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		SYNJ1_HUMAN (10-466): 1.18e-22: 104: 11.71
+	Hs
		655524 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		MYO16_HUMAN (111-438): 6.65e-11: 67.4: 12.90
+	Hs
		412433 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		MYO16_HUMAN (111-438): 6.65e-11: 67.4: 12.90
+	Hs
		522749 SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32		MUC1_HUMAN (87-469): 7.93e-15: 79.7: 9.92