HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: GAB1 (Q13480)
GRB2-associated-binding protein 1 (GRB2-associated binder 1) (Growth factor receptor bound protein 2-associated protein 1)
Gene: GAB1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.692

Property	Details
Experiments	24728074
Identification Methods	pull down, fluorescence polarization spectroscopy
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	PH~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005911 - cell-cell junction GO:0007165 - signal transduction GO:0035591 - signaling adaptor activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 694
+	Hs
		209049 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Hs
		557834 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		GAB2_HUMAN (3-691): 2.24e-153: 461: 39.01
+	Hs
		788991 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Hs
		655356 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Hs
		129290 P85B_HUMAN (100-376): 1.85e-21: 98.3: 16.61		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Mm
		643113 P85A_MOUSE (241-376): 1.59e-22: 101: 19.57		GAB1_MOUSE (1-694): 0: 544: 89.93
+	Hs
		442825 HCK_HUMAN (2-174): 4.07e-20: 93.7: 17.24		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Hs
		522965 SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32		GAB1_HUMAN (1-694): 0: 547: 100.00
+	Mm
		403466 SRC_MOUSE (5-114): 1.55e-15: 79.7: 22.32		GAB1_MOUSE (1-694): 0: 544: 89.93
+	Hs
		1040322 GRAP_HUMAN (5-251): 9.35e-27: 107: 20.00		GAB2_HUMAN (3-691): 2.24e-153: 461: 39.01
+	Hs
		655510 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		GAB2_HUMAN (3-691): 2.24e-153: 461: 39.01
+	Hs
		129351 P85B_HUMAN (100-376): 1.85e-21: 98.3: 16.61		GAB2_HUMAN (3-691): 2.24e-153: 461: 39.01
+	Hs
		523213 SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32		GAB2_HUMAN (3-691): 2.24e-153: 461: 39.01
+	Mm
		643089 P85A_MOUSE (241-376): 1.59e-22: 101: 19.57		Q3ZB59_MOUSE (3-691): 1.2e-140: 428: 38.33
+	Mm
		140067 P85B_MOUSE (252-376): 2.57e-21: 97.5: 24.41		GAB2_MOUSE (3-691): 8.7e-141: 429: 38.33
+	Mm
		140061 P85B_MOUSE (252-376): 2.57e-21: 97.5: 24.41		Q3ZB59_MOUSE (3-691): 1.2e-140: 428: 38.33
+	Mm
		1114082 GRB2_MOUSE (1-61): 1.55e-16: 79.4: 29.03		Q3ZB59_MOUSE (3-691): 1.2e-140: 428: 38.33
+	Hs
		412280 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		GAB3_HUMAN (1-683): 1.81e-103: 329: 28.82
+	Hs
		523086 SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32		GAB3_HUMAN (1-683): 1.81e-103: 329: 28.82
+	Dm
		993221 GRAP_DROME (1-61): 1.53e-17: 82.3: 31.15		DOS_DROME (7-655): 6.26e-82: 279: 18.50