HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: DAG1 (Q14118)
Dystroglycan 1 (Dystroglycan) (Dystrophin-associated glycoprotein 1) [Cleaved into: Alpha-dystroglycan (Alpha-DG); Beta-dystroglycan (Beta-DG)]
Gene: DAG1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.64	0.60	0.624

Property	Details
Experiments	11724572, 17474147
Identification Methods	pull down, affinity chromatography technology, peptide array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 377 Hs			1 895
+	Hs
		655363 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		DAG1_HUMAN (1-895): 7.39e-154: 476: 100.00
+	Oc
		101982 G1SR27_RABIT (3-61): 2.12e-16: 79.1: 28.81		DAG1_RABIT (1-895): 9.3e-162: 497: 93.74
+	Hs
		412189 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		DAG1_HUMAN (1-895): 7.39e-154: 476: 100.00
+	Hs
		522977 SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32		DAG1_HUMAN (1-895): 7.39e-154: 476: 100.00
+	Hs
		924561 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		DAG1_HUMAN (1-895): 7.39e-154: 476: 100.00
+	Hs
		782787 CSK_HUMAN (4-143): 1.92e-12: 70.2: 14.38		DAG1_HUMAN (1-895): 7.39e-154: 476: 100.00
+	Hs
		1048511 SH3K1_HUMAN (58-207): 2.14e-09: 61.3: 17.76		DAG1_HUMAN (1-895): 7.39e-154: 476: 100.00
+	Hs
		203208 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		SYNJ1_HUMAN (180-822): 3.46e-23: 108: 13.52
+	Hs
		203192 SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26		SYNJ1_HUMAN (180-822): 3.46e-23: 108: 13.52
+	Hs
		522749 SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32		MUC1_HUMAN (67-890): 1.18e-26: 119: 9.42
+	Rn
		192744 SRBS2_RAT (3-162): 4.86e-25: 109: 28.05		SYNJ1_RAT (180-750): 2.69e-17: 89.6: 12.27
+	Hs
		203230 SH319_HUMAN (8-254): 6.26e-19: 90.5: 19.84		SYNJ1_HUMAN (180-822): 3.46e-23: 108: 13.52
+	Hs
		554292 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		MUC1_HUMAN (67-890): 1.18e-26: 119: 9.42
+	Hs
		203228 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		SYNJ1_HUMAN (180-822): 3.46e-23: 108: 13.52
+	Hs
		203218 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		SYNJ1_HUMAN (180-822): 3.46e-23: 108: 13.52
+	Mm
		101430 CSK_MOUSE (4-143): 1.65e-12: 70.2: 14.38		F8VPR5_MOUSE (175-582): 1.7e-22: 106: 11.28
+	Hs
		203241 SH3K1_HUMAN (58-207): 2.14e-09: 61.3: 17.76		SYNJ1_HUMAN (180-822): 3.46e-23: 108: 13.52
+	Hs
		924760 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		PRG4_HUMAN (350-894): 1.68e-13: 76.9: 13.21
+	Hs
		1056926 SH3K1_HUMAN (58-207): 2.14e-09: 61.3: 17.76		MDC1_HUMAN (98-885): 3e-18: 92.7: 11.71