HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: ABI1 (Q8IZP0)
Abl interactor 1 (Abelson interactor 1) (Abi-1) (Abl-binding protein 4) (AblBP4) (Eps8 SH3 domain-binding protein) (Eps8-binding protein) (Nap1-binding protein) (Nap1BP) (Spectrin SH3 domain-binding protein 1) (e3B1)
Gene: ABI1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	33961781, 11418237, 11557983
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005634 - nucleus GO:0005783 - endoplasmic reticulum GO:0005829 - cytosol GO:0035591 - signaling adaptor activity GO:0045296 - cadherin binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 508
+	Hs
		209108 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ABI1_HUMAN (1-508): 3.82e-124: 374: 100.00
+	Mm
		223016 NCK2_MOUSE (1-376): 3.75e-90: 277: 66.93		ABI1_MOUSE (1-508): 1.42e-122: 369: 91.93
+	Hs
		557806 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ABI2_HUMAN (1-508): 1.41e-81: 264: 59.84
+	Hs
		209180 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ABI2_HUMAN (1-508): 1.41e-81: 264: 59.84
+	Hs
		789078 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		ABI1_HUMAN (1-508): 3.82e-124: 374: 100.00
+	Hs
		203208 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		SYNJ1_HUMAN (38-463): 1.36e-41: 161: 11.55
+	Hs
		557823 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ASAP1_HUMAN (107-505): 8.23e-33: 135: 16.83
+	Rn
		71363 B2RZ33_RAT (1-377): 8.61e-99: 300: 99.20		SRC8_RAT (291-502): 1.57e-31: 129: 19.73
+	Hs
		166030 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		SYNJ2_HUMAN (39-458): 2.1e-26: 115: 13.88
+	Hs
		209187 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ASAP1_HUMAN (107-505): 8.23e-33: 135: 16.83
+	Hs
		789094 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		ABI2_HUMAN (1-508): 1.41e-81: 264: 59.84
+	Hs
		235470 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		VINEX_HUMAN (229-504): 5.02e-15: 79.8: 15.58
+	Hs
		3146 A0A0S2Z4E4_HUMAN (1-181): 3.34e-26: 107: 74.87		ABI2_HUMAN (1-508): 1.41e-81: 264: 59.84
+	Hs
		209184 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ABI3_HUMAN (250-502): 1.37e-16: 83.3: 25.48
+	Hs
		789012 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		ASAP1_HUMAN (107-505): 8.23e-33: 135: 16.83
+	Hs
		789066 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		SRC8_HUMAN (291-502): 1.23e-34: 138: 20.72
+	Rn
		192744 SRBS2_RAT (3-162): 4.86e-25: 109: 28.05		SYNJ1_RAT (38-459): 5.26e-40: 157: 12.39
+	Hs
		203192 SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26		SYNJ1_HUMAN (38-463): 1.36e-41: 161: 11.55
+	Hs
		235483 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		VINEX_HUMAN (229-504): 5.02e-15: 79.8: 15.58
+	Hs
		298986 SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26		SH3R1_HUMAN (244-503): 3.15e-09: 61.6: 17.28