HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: WASF1 (Q92558)
Actin-binding protein WASF1 (Protein WAVE-1) (Verprolin homology domain-containing protein 1) (Wiskott-Aldrich syndrome protein family member 1) (WASP family protein member 1)
Gene: WASF1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	0.60	0.536

Property	Details
Experiments	33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 559
+	Hs
		209133 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		WASF1_HUMAN (1-559): 2.63e-73: 245: 100.00
+	Hs
		557843 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		WASF2_HUMAN (1-541): 2.37e-42: 160: 42.14
+	Hs
		209188 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		WASF3_HUMAN (1-557): 1.12e-43: 164: 45.73
+	Hs
		209196 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		WASF2_HUMAN (1-541): 2.37e-42: 160: 42.14
+	Hs
		166030 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		SYNJ2_HUMAN (46-507): 2.06e-31: 132: 13.92
+	Hs
		557670 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49
+	Mm
		222878 NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94		ACK1_MOUSE (162-530): 2.06e-21: 100: 18.09
+	Hs
		209039 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49
+	Hs
		203208 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		SYNJ1_HUMAN (41-523): 2.02e-11: 69: 12.53
+	Hs
		298991 SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26		WASF1_HUMAN (1-559): 2.63e-73: 245: 100.00
+	Hs
		655430 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		WASF1_HUMAN (1-559): 2.63e-73: 245: 100.00
+	Mm
		643108 P85A_MOUSE (241-376): 1.59e-22: 101: 19.57		WASF1_MOUSE (1-559): 8.6e-72: 241: 98.39
+	Rn
		192742 SRBS2_RAT (3-162): 4.86e-25: 109: 28.05		Q5FWU0_RAT (1-291): 5.25e-59: 206: 52.58
+	Ce
		712432 Q95PW9_CAEEL (1-376): 2.62e-70: 227: 38.69		SC161_CAEEL (208-492): 3.01e-09: 62: 14.97
+	Rn
		192744 SRBS2_RAT (3-162): 4.86e-25: 109: 28.05		SYNJ1_RAT (123-554): 5.15e-35: 142: 12.99
+	Hs
		166032 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		SYNJ2_HUMAN (46-507): 2.06e-31: 132: 13.92
+	Hs
		655342 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		EP300_HUMAN (77-554): 1.1e-28: 124: 14.91
+	Mm
		70897 P85A_MOUSE (241-376): 1.59e-22: 101: 19.57		EP300_MOUSE (77-554): 6.02e-27: 118: 13.31
+	Hs
		655334 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49
+	Hs
		203192 SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26		SYNJ1_HUMAN (41-523): 2.02e-11: 69: 12.53