HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: SPN90 (Q9NZQ3)
NCK-interacting protein with SH3 domain (54 kDa VacA-interacting protein) (54 kDa vimentin-interacting protein) (VIP54) (90 kDa SH3 protein interacting with Nck) (AF3p21) (Dia-interacting protein 1) (DIP-1) (Diaphanous protein-interacting protein) (SH3 adapter protein SPIN90) (WASP-interacting SH3-domain protein) (WISH) (Wiskott-Aldrich syndrome protein-interacting protein)
Gene: NCKIPSD

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.77	1.00	0.882

Property	Details
Experiments	28514442, 33961781, 11278500
Identification Methods	affinity chromatography technology, pull down, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0010976 - positive regulation of neuron projection development
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1 377 Hs			1 722
+	Hs
		209182 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		412383 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		1078920 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		924910 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		1349276 ITSN2_HUMAN (7-85): 2.68e-12: 70.6: 24.05		SPN90_HUMAN (1-722): 0: 1137: 100.00