HitPredict

Interactor 1: GBB3 (P16520)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 (Transducin beta chain 3)
Gene: GNB3

Interactor 2: GBG5 (P63218)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-5
Gene: GNG5

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.82	1.00	0.907

Property	Details
Experiments	33961781, 8636150, 19168127, 22940628
Identification Methods	affinity chromatography technology, two hybrid, pull down, fluorescent resonance energy transfer, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	G-gamma~WD40
Common GO terms	GO:0003924 - GTPase activity GO:0005834 - heterotrimeric G-protein complex GO:0005886 - plasma membrane GO:0007186 - G protein-coupled receptor signaling pathway GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 340 Hs			1 68
+	Hs
		229235 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG7_HUMAN (1-68): 1.09e-27: 96.2: 51.47
+	Hs
		560893 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG12_HUMAN (2-68): 5.4e-26: 92: 46.27
+	Hs
		560868 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG2_HUMAN (2-68): 6.55e-26: 91.6: 48.53
+	Hs
		560872 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG3_HUMAN (1-68): 1.3e-25: 90.9: 46.38
+	Hs
		229238 GBB2_HUMAN (1-340): 2.98e-103: 308: 78.49		GBG7_HUMAN (1-68): 1.09e-27: 96.2: 51.47
+	Hs
		560865 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG10_HUMAN (1-68): 5.93e-25: 89.3: 52.94
+	Hs
		560864 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG4_HUMAN (2-68): 1.64e-24: 88.2: 45.59
+	Hs
		560895 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG8_HUMAN (2-68): 1.13e-23: 85.8: 44.12
+	Hs
		887097 GBB2_HUMAN (1-340): 2.98e-103: 308: 78.49		GBG2_HUMAN (2-68): 6.55e-26: 91.6: 48.53
+	Hs
		921516 GBB2_HUMAN (1-340): 2.98e-103: 308: 78.49		GBG3_HUMAN (1-68): 1.3e-25: 90.9: 46.38
+	Hs
		837687 GBB2_HUMAN (1-340): 2.98e-103: 308: 78.49		GBG10_HUMAN (1-68): 5.93e-25: 89.3: 52.94
+	Hs
		837673 GBB2_HUMAN (1-340): 2.98e-103: 308: 78.49		GBG4_HUMAN (2-68): 1.64e-24: 88.2: 45.59
+	Hs
		921517 GBB2_HUMAN (1-340): 2.98e-103: 308: 78.49		GBG5_HUMAN (1-68): 5.15e-24: 86.6: 100.00
+	Hs
		560892 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG13_HUMAN (7-68): 9.95e-21: 78.5: 25.81
+	Hs
		150112 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBGT2_HUMAN (10-67): 1.31e-18: 73.1: 31.03
+	Hs
		560869 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG11_HUMAN (9-67): 1.67e-18: 73.1: 32.20
+	Hs
		560871 GBB3_HUMAN (1-340): 9.93e-106: 314: 100.00		GBG1_HUMAN (9-67): 1.93e-18: 72.7: 28.33
+	Hs
		150115 GBB2_HUMAN (1-340): 2.98e-103: 308: 78.49		GBGT2_HUMAN (10-67): 1.31e-18: 73.1: 31.03
+	Hs
		921515 GBB2_HUMAN (1-340): 2.98e-103: 308: 78.49		GBG1_HUMAN (9-67): 1.93e-18: 72.7: 28.33
+	Hs
		929139 Q6FHM2_HUMAN (1-340): 2.98e-103: 308: 78.49		GBG1_HUMAN (9-67): 1.93e-18: 72.7: 28.33