HitPredict

Interactor 1: BTF3 (P20290)
Transcription factor BTF3 (Nascent polypeptide-associated complex subunit beta) (NAC-beta) (RNA polymerase B transcription factor 3)
Gene: BTF3

Interactor 2: NACA (Q13765)
Nascent polypeptide-associated complex subunit alpha (NAC-alpha) (Alpha-NAC) (allergen Hom s 2)
Gene: NACA

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.971

Property	Details
Experiments	10982809, 21203952, 30948508, 24981860, 37071682, 33961781, 33644029, 35271311, 35235311, 34349018
Identification Methods	pull down, x-ray crystallography, affinity chromatography technology, cross-linking study, anti tag coimmunoprecipitation
Interaction Type	direct interaction, association, physical association

Property	Details
Interacting Pfam domains	NAC~NAC
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005854 - nascent polypeptide-associated complex GO:0015031 - protein transport GO:1905551 - negative regulation of protein localization to endoplasmic reticulum
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 206 Hs			1 215
+	Hs
		591093 BTF3_HUMAN (1-206): 3.5e-94: 275: 100.00		NACA2_HUMAN (1-215): 4.72e-93: 272: 90.23
+	Hs
		166496 BTF3_HUMAN (1-206): 3.5e-94: 275: 100.00		NACAD_HUMAN (4-215): 3.39e-72: 239: 61.64
+	Hs
		1295755 BT3L4_HUMAN (50-206): 5.07e-67: 204: 81.01		NACA2_HUMAN (1-215): 4.72e-93: 272: 90.23
+	Hs
		1045198 BT3L4_HUMAN (50-206): 5.07e-67: 204: 81.01		NACA_HUMAN (1-215): 5.13e-88: 260: 100.00
+	Hs
		166511 BT3L4_HUMAN (50-206): 5.07e-67: 204: 81.01		NACAD_HUMAN (4-215): 3.39e-72: 239: 61.64
+	Dm
		1180829 Q7KM15_DROME (50-201): 3.3e-57: 179: 63.40		NACA_DROME (6-215): 4.42e-71: 217: 61.32
+	Ce
		1090365 BTF3_CAEEL (53-204): 4.88e-59: 184: 63.16		NACA_CAEEL (6-215): 1.21e-67: 207: 54.29
+	Dm
		1180836 Q7KM15_DROME (50-201): 3.3e-57: 179: 63.40		Q9VPV7_DROME (6-215): 3.3e-62: 198: 37.50
+	Dm
		1276591 Q9VPR2_DROME (50-205): 6.66e-44: 157: 59.87		NACA_DROME (6-215): 4.42e-71: 217: 61.32
+	Dm
		1372034 Q9VPR2_DROME (50-205): 6.66e-44: 157: 59.87		Q9VPV7_DROME (6-215): 3.3e-62: 198: 37.50
+	Dm
		1276606 Q9VY34_DROME (50-136): 4.56e-27: 105: 35.63		NACA_DROME (6-215): 4.42e-71: 217: 61.32
+	Dm
		71736 B3DNI1_DROME (50-136): 1.25e-26: 104: 36.78		NACA_DROME (6-215): 4.42e-71: 217: 61.32
+	Dm
		1001359 Q0IGS9_DROME (50-136): 2.9e-26: 103: 34.48		NACA_DROME (6-215): 4.42e-71: 217: 61.32
+	Dm
		1276607 Q9VY63_DROME (50-136): 3.25e-26: 103: 36.78		NACA_DROME (6-215): 4.42e-71: 217: 61.32
+	Pf
		86939 Q8ILK2_PLAF7 (47-203): 3.43e-41: 139: 32.93		C6KT55_PLAF7 (63-214): 2.56e-56: 178: 33.77
+	Sc
		756709 NACB1_YEAST (50-199): 3.71e-48: 156: 36.13		NACA_YEAST (62-214): 8.33e-46: 151: 34.12
+	Dm
		1372155 Q9VY34_DROME (50-136): 4.56e-27: 105: 35.63		Q9VPV7_DROME (6-215): 3.3e-62: 198: 37.50
+	Dm
		71738 B3DNI1_DROME (50-136): 1.25e-26: 104: 36.78		Q9VPV7_DROME (6-215): 3.3e-62: 198: 37.50
+	Dm
		1001360 Q0IGS9_DROME (50-136): 2.9e-26: 103: 34.48		Q9VPV7_DROME (6-215): 3.3e-62: 198: 37.50
+	Sc
		756680 NACB2_YEAST (50-172): 2.41e-43: 144: 34.92		NACA_YEAST (62-214): 8.33e-46: 151: 34.12