Interactor 1: RPB4 (P20433)
DNA-directed RNA polymerase II subunit RPB4 (RNA polymerase II subunit B4) (B32) (DNA-directed RNA polymerase II 32 kDa polypeptide)
Gene: RPB4

Interactor 2: RPB7 (P34087)
DNA-directed RNA polymerase II subunit RPB7 (RNA polymerase II subunit B7) (B16)
Gene: RPB7

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.98 1.00 0.992

The method score is based on:

Property Details
Experiments 9545247, 7579693, 18552824, 1985924, 17937913, 16964259, 19820686, 11805826, 12697831, 10898792, 12746498, 12746495, 15591044, 11087726, 15913559, 25315781, 33440147, 21074047, 21406554, 14530281 and 10 others

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Identification Methods electron microscopy, two hybrid, molecular sieving, biochemical, mass spectrometry studies of complexes, pull down, tandem affinity purification, x-ray crystallography, affinity chromatography technology
Interaction Type direct interaction, association, physical association

The annotation score is based on:

Property Details
Interacting Pfam domains RNA_pol_Rpb4~S1, RNA_pol_Rpb4~SHS2_Rpb7-N
Common GO terms GO:0000288 - nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
GO:0000932 - P-body
GO:0001172 - RNA-templated transcription
GO:0005634 - nucleus
GO:0005665 - RNA polymerase II, core complex
and 8 others

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Homologous interactions 4

Homologous interactions

Color coding for hit scores

1 221
Sc

1 171
+ Sp
+ Dm
+ Hs
+ Hs

Service provided by Combinatics, Japan

Last updated: 15 Jul 2024