HitPredict

Interactor 1: UBA1 (P22314)
Ubiquitin-like modifier-activating enzyme 1 (EC 6.2.1.45) (Protein A1S9) (Ubiquitin-activating enzyme E1)
Gene: UBA1

Interactor 2: UB2J1 (Q9Y385)
Ubiquitin-conjugating enzyme E2 J1 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme J1) (Non-canonical ubiquitin-conjugating enzyme 1) (NCUBE-1) (Yeast ubiquitin-conjugating enzyme UBC6 homolog E) (HsUBC6e)
Gene: UBE2J1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.69	1.00	0.835

Property	Details
Experiments	31518855, 17597759, 19549727
Identification Methods	enzymatic study, pull down, two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	E1_UFD~UQ_con, ThiF~UQ_con
Common GO terms	GO:0005524 - ATP binding GO:0005634 - nucleus
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1058 Hs			1 318
+	Hs
		607050 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2D2_HUMAN (10-156): 5.15e-70: 216: 28.00
+	Hs
		607190 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2D4_HUMAN (10-156): 3.97e-70: 216: 28.00
+	Hs
		607026 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2D1_HUMAN (10-156): 1e-69: 215: 30.00
+	Hs
		607039 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2D3_HUMAN (10-156): 1.73e-68: 212: 28.00
+	Hs
		607028 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2E1_HUMAN (1-156): 1.67e-64: 203: 28.30
+	Hs
		607112 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2J2_HUMAN (1-150): 3.16e-63: 202: 39.87
+	Hs
		607129 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2E3_HUMAN (2-156): 9.16e-63: 199: 26.58
+	Hs
		607136 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2E2_HUMAN (2-156): 2.19e-62: 198: 27.22
+	Hs
		607023 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UBE2A_HUMAN (7-154): 3.69e-61: 193: 21.85
+	Hs
		607054 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UBE2B_HUMAN (7-154): 6.08e-60: 190: 22.52
+	Hs
		607038 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2G2_HUMAN (7-142): 5.84e-58: 185: 26.00
+	Hs
		607046 UBA1_HUMAN (1-1058): 0: 1358: 100.00		UB2G1_HUMAN (15-148): 2.26e-56: 182: 25.83
+	Sp
		293848 UBA1_SCHPO (36-1053): 0: 1080: 53.37		UBC4_SCHPO (10-155): 6.78e-69: 213: 28.19
+	Sp
		193850 UBA1_SCHPO (36-1053): 0: 1080: 53.37		UBC6_SCHPO (8-148): 1.55e-59: 192: 40.85
+	Sc
		549788 UBA1_YEAST (38-1057): 0: 1063: 51.50		UBC4_YEAST (9-156): 2.23e-66: 206: 25.83
+	Sp
		293847 UBA1_SCHPO (36-1053): 0: 1080: 53.37		UBC2_SCHPO (7-146): 6.12e-59: 187: 20.98
+	Sc
		608684 UBA1_YEAST (38-1057): 0: 1063: 51.50		UBC6_YEAST (9-148): 3.01e-61: 197: 39.72
+	Hs
		48104 UBA6_HUMAN (12-1057): 0: 1037: 41.09		UB2D1_HUMAN (10-156): 1e-69: 215: 30.00
+	Hs
		48105 UBA6_HUMAN (12-1057): 0: 1037: 41.09		UB2G2_HUMAN (7-142): 5.84e-58: 185: 26.00
+	Sp
		142467 UBA1_SCHPO (36-1053): 0: 1080: 53.37		UBC13_SCHPO (13-119): 5.55e-15: 72.8: 40.91