HitPredict

Interactor 1: PRP22 (P24384)
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 (EC 3.6.4.13)
Gene: PRP22

Interactor 2: CEF1 (Q03654)
Pre-mRNA-splicing factor CEF1 (PRP nineteen-associated complex protein 85) (PRP19-associated complex protein 85)
Gene: CEF1

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.86	0.60	0.720

Property	Details
Experiments	11805826, 29146870, 20089683, 11884590, 16429126
Identification Methods	tandem affinity purification, pull down, affinity chromatography technology
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000350 - generation of catalytic spliceosome for second transesterification step
Homologous interactions	11

Homologous interactions

Color coding for hit scores

	1 1145 Sc			1 590
+	Sc
		589450 PRP2_YEAST (252-1113): 0: 837: 34.18		CEF1_YEAST (1-590): 5.79e-164: 482: 100.00
+	Hs
		1055461 DHX8_HUMAN (1-1137): 0: 949: 40.81		CDC5L_HUMAN (1-537): 2.19e-100: 324: 29.51
+	Sp
		193838 PRP22_SCHPO (1-1138): 0: 918: 44.27		CEF1_SCHPO (8-531): 2.5e-85: 283: 27.98
+	Sc
		859420 PRP43_YEAST (416-1106): 0: 700: 45.80		CEF1_YEAST (1-590): 5.79e-164: 482: 100.00
+	Sc
		554244 PRP16_YEAST (217-1109): 0: 670: 35.31		CEF1_YEAST (1-590): 5.79e-164: 482: 100.00
+	Hs
		1220267 DHX40_HUMAN (433-1130): 0: 826: 37.62		CDC5L_HUMAN (1-537): 2.19e-100: 324: 29.51
+	Hs
		1268330 PRP16_HUMAN (105-1139): 0: 809: 35.69		CDC5L_HUMAN (1-537): 2.19e-100: 324: 29.51
+	Hs
		224835 DHX16_HUMAN (242-1114): 0: 780: 41.22		CDC5L_HUMAN (1-537): 2.19e-100: 324: 29.51
+	Sp
		765721 PRP16_SCHPO (3-1145): 0: 819: 34.14		CEF1_SCHPO (8-531): 2.5e-85: 283: 27.98
+	Sp
		765714 CDC28_SCHPO (4-1113): 0: 762: 32.50		CEF1_SCHPO (8-531): 2.5e-85: 283: 27.98
+	Sp
		194925 DHX15_SCHPO (410-1129): 0: 716: 44.82		CEF1_SCHPO (8-531): 2.5e-85: 283: 27.98