HitPredict

Interactor 1: APC (P25054)
Adenomatous polyposis coli protein (Protein APC) (Deleted in polyposis 2.5)
Gene: APC

Interactor 2: ARHG4 (Q9NR80)
Rho guanine nucleotide exchange factor 4 (APC-stimulated guanine nucleotide exchange factor 1) (Asef) (Asef1)
Gene: ARHGEF4

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.82	0.60	0.703

Property	Details
Experiments	10947987, 33961781, 22000517, 17145773
Identification Methods	pull down, affinity chromatography technology, isothermal titration calorimetry, two hybrid, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0032587 - ruffle membrane
Homologous interactions	6

Homologous interactions

Color coding for hit scores

	1 2843 Hs			1 690
+	Hs
		626231 APC_HUMAN (1-2843): 0: 1316: 100.00		SPT13_HUMAN (95-690): 1.64e-140: 427: 57.24
+	Rn
		88807 APC_RAT (1-2843): 0: 1222: 89.60		D3ZWB4_RAT (94-690): 3.04e-129: 414: 57.31
+	Hs
		257713 ANR17_HUMAN (1869-2843): 8.13e-62: 238: 14.08		ITSN2_HUMAN (5-680): 2.13e-102: 345: 16.45
+	Hs
		1301019 CIC_HUMAN (722-2643): 2.1e-17: 92.4: 7.79		FARP1_HUMAN (50-661): 3.43e-75: 263: 16.51
+	Mm
		57869 ANK2_MOUSE (1056-2841): 1.1e-15: 86.5: 9.74		KALRN_MOUSE (273-670): 1.33e-54: 205: 19.70
+	Hs
		855816 TITIN_HUMAN (717-2430): 7.06e-29: 130: 7.55		VAV2_HUMAN (4-248): 7.82e-20: 96.6: 11.64