HitPredict

Interactor 1: RHG32 (A7KAX9)
Rho GTPase-activating protein 32 (Brain-specific Rho GTPase-activating protein) (GAB-associated Cdc42/Rac GTPase-activating protein) (GC-GAP) (GTPase regulator interacting with TrkA) (Rho-type GTPase-activating protein 32) (Rho/Cdc42/Rac GTPase-activating protein RICS) (RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling) (p200RhoGAP) (p250GAP)
Gene: ARHGAP32

Interactor 2: CDC42 (P60953)
Cell division control protein 42 homolog (EC 3.6.5.2) (G25K GTP-binding protein)
Gene: CDC42

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.85	1.00	0.922

Property	Details
Experiments	12857875, 12819203, 12446789, 31871319, 20936779
Identification Methods	enzymatic study, affinity chromatography technology, two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Ras~RhoGAP
Common GO terms	GO:0000139 - Golgi membrane GO:0005789 - endoplasmic reticulum membrane GO:0005829 - cytosol GO:0043197 - dendritic spine
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 2087 Hs			1 191
+	Hs
		64652 RHG32_HUMAN (1-2087): 0: 1424: 100.00		RHOQ_HUMAN (4-187): 7.35e-91: 266: 67.94
+	Hs
		64741 RHG32_HUMAN (1-2087): 0: 1424: 100.00		RHOJ_HUMAN (2-188): 2.67e-85: 252: 64.17
+	Hs
		64688 RHG32_HUMAN (1-2087): 0: 1424: 100.00		RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31
+	Hs
		64692 RHG32_HUMAN (1-2087): 0: 1424: 100.00		RHOG_HUMAN (1-191): 7.59e-82: 242: 60.73
+	Hs
		64681 RHG32_HUMAN (1-2087): 0: 1424: 100.00		RHOA_HUMAN (5-191): 3.88e-81: 240: 53.48
+	Hs
		64649 RHG32_HUMAN (1-2087): 0: 1424: 100.00		RAC2_HUMAN (1-191): 1.49e-80: 239: 68.75
+	Hs
		64644 RHG32_HUMAN (1-2087): 0: 1424: 100.00		RHOC_HUMAN (5-191): 3.64e-79: 235: 52.41
+	Hs
		64679 RHG32_HUMAN (1-2087): 0: 1424: 100.00		RAC3_HUMAN (1-177): 2.36e-78: 233: 72.32
+	Mm
		925803 RHG32_MOUSE (1-2087): 0: 1404: 87.28		RAC1_MOUSE (1-191): 1.75e-84: 249: 70.31
+	Mm
		892730 RHG32_MOUSE (1-2087): 0: 1404: 87.28		CDC42_MOUSE (1-191): 4.96e-82: 243: 100.00
+	Mm
		1194500 RHG33_MOUSE (73-1289): 3.48e-114: 398: 30.65		RHOJ_MOUSE (2-179): 1.33e-84: 250: 65.17
+	Hs
		147862 RHG33_HUMAN (74-1276): 2.2e-113: 395: 30.98		RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31
+	Mm
		892729 RHG33_MOUSE (73-1289): 3.48e-114: 398: 30.65		CDC42_MOUSE (1-191): 4.96e-82: 243: 100.00
+	Dm
		776916 CDGAP_DROME (76-1527): 1.83e-93: 339: 20.94		RAC1_DROME (1-191): 1.02e-81: 242: 69.27
+	Hs
		925314 RHG44_HUMAN (335-893): 3.16e-80: 286: 19.37		RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31
+	Hs
		200213 SRGP3_HUMAN (294-939): 5.72e-78: 284: 17.36		RAC1_HUMAN (1-191): 1.75e-84: 249: 70.31
+	Hs
		894068 RHG44_HUMAN (335-893): 3.16e-80: 286: 19.37		CDC42_HUMAN (1-191): 4.96e-82: 243: 100.00
+	Hs
		893974 MYO9B_HUMAN (43-823): 2.74e-75: 281: 13.14		CDC42_HUMAN (1-191): 4.96e-82: 243: 100.00
+	Hs
		900443 MYO9B_HUMAN (43-823): 2.74e-75: 281: 13.14		RHOA_HUMAN (5-191): 3.88e-81: 240: 53.48
+	Hs
		436289 MYO9B_HUMAN (43-823): 2.74e-75: 281: 13.14		RHOC_HUMAN (5-191): 3.64e-79: 235: 52.41