HitPredict

Interactor 1: AP2B (P27351)
AP-2 complex subunit beta (Beta-2-adaptin) (Beta-adaptin) (Clathrin assembly protein complex 2 beta large chain) (Clathrin assembly protein large beta chain)
Gene: APL1

Interactor 2: AP2M (Q99186)
AP-2 complex subunit mu (Adaptin medium chain APM4) (Clathrin assembly protein complex 2 mu medium chain) (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) (Mu2-adaptin) (Plasma membrane adaptor AP-2 50 kDa protein)
Gene: APM4

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.85	1.00	0.926

Property	Details
Experiments	10564262, 25346786, 16429126, 19822444, 37968396
Identification Methods	coimmunoprecipitation, two hybrid, affinity chromatography technology, anti tag coimmunoprecipitation, tandem affinity purification
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Adap_comp_sub~Adaptin_N
Common GO terms	GO:0006886 - intracellular protein transport GO:0030122 - AP-2 adaptor complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 700 Sc			1 491
+	Hs
		925992 AP2B1_HUMAN (1-699): 0: 583: 27.89		AP1M1_HUMAN (2-490): 7.21e-162: 466: 28.05
+	Hs
		925969 AP2B1_HUMAN (1-699): 0: 583: 27.89		AP2M1_HUMAN (1-490): 2.06e-154: 448: 30.52
+	Hs
		926066 AP2B1_HUMAN (1-699): 0: 583: 27.89		AP1M2_HUMAN (2-490): 1.09e-154: 448: 28.25
+	Hs
		1014074 AP1B1_HUMAN (1-700): 0: 556: 26.69		AP1M1_HUMAN (2-490): 7.21e-162: 466: 28.05
+	At
		187750 APBLC_ARATH (3-700): 0: 559: 28.10		AP2M_ARATH (2-490): 2.05e-156: 453: 30.08
+	At
		187886 APBLB_ARATH (3-699): 0: 553: 27.71		AP2M_ARATH (2-490): 2.05e-156: 453: 30.08
+	Hs
		1014061 AP1B1_HUMAN (1-700): 0: 556: 26.69		AP2M1_HUMAN (1-490): 2.06e-154: 448: 30.52
+	Hs
		1014107 AP1B1_HUMAN (1-700): 0: 556: 26.69		AP1M2_HUMAN (2-490): 1.09e-154: 448: 28.25
+	Ce
		725358 Q9N4F3_CAEEL (1-657): 0: 543: 28.29		AP1M_CAEEL (3-490): 4.45e-153: 444: 28.51
+	Ce
		725366 Q9N4F3_CAEEL (1-657): 0: 543: 28.29		AP2M_CAEEL (1-490): 6.21e-153: 444: 30.52
+	Dm
		248295 Q24253_DROME (1-634): 0: 548: 28.64		O62530_DROME (1-490): 5.33e-150: 436: 31.73
+	Mm
		612130 AP1G1_MOUSE (5-692): 8.86e-165: 496: 11.38		AP1M1_MOUSE (2-490): 3.21e-161: 465: 27.85
+	Hs
		212662 AP1G1_HUMAN (5-692): 3.91e-164: 494: 11.38		AP1M1_HUMAN (2-490): 7.21e-162: 466: 28.05
+	Hs
		212655 AP1G1_HUMAN (5-692): 3.91e-164: 494: 11.38		AP2M1_HUMAN (1-490): 2.06e-154: 448: 30.52
+	Hs
		212676 AP1G1_HUMAN (5-692): 3.91e-164: 494: 11.38		AP1M2_HUMAN (2-490): 1.09e-154: 448: 28.25
+	At
		187823 AP2A1_ARATH (1-692): 3.82e-151: 466: 13.06		AP2M_ARATH (2-490): 2.05e-156: 453: 30.08
+	Hs
		819043 COPG2_HUMAN (2-698): 1.54e-161: 489: 13.34		COPD_HUMAN (7-489): 2.78e-127: 381: 13.37
+	Hs
		819054 COPG1_HUMAN (3-698): 1.11e-157: 479: 13.46		COPD_HUMAN (7-489): 2.78e-127: 381: 13.37
+	Hs
		925959 AP2B1_HUMAN (1-699): 0: 583: 27.89		STON2_HUMAN (124-489): 5.92e-82: 274: 18.83
+	Hs
		1014058 AP1B1_HUMAN (1-700): 0: 556: 26.69		STON2_HUMAN (124-489): 5.92e-82: 274: 18.83