HitPredict

Interactor 1: MARK3 (P27448)
MAP/microtubule affinity-regulating kinase 3 (EC 2.7.11.1) (C-TAK1) (cTAK1) (Cdc25C-associated protein kinase 1) (ELKL motif kinase 2) (EMK-2) (Protein kinase STK10) (Ser/Thr protein kinase PAR-1) (Par-1a) (Serine/threonine-protein kinase p78)
Gene: MARK3

Interactor 2: NUAK2 (Q9H093)
NUAK family SNF1-like kinase 2 (EC 2.7.11.1) (Omphalocele kinase 2) (SNF1/AMP kinase-related kinase) (SNARK)
Gene: NUAK2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	1.00	0.811

Property	Details
Experiments	28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	KA1~Pkinase, Pkinase~Pkinase, Pkinase~UBA
Common GO terms	GO:0004674 - protein serine/threonine kinase activity GO:0005524 - ATP binding GO:0005737 - cytoplasm GO:0006468 - protein phosphorylation GO:0035556 - intracellular signal transduction and 1 others more... GO:0106310 - protein serine kinase activity
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 753 Hs			1 628
+	Hs
		647915 MARK3_HUMAN (1-753): 0: 769: 100.00		MARK2_HUMAN (6-613): 9.9e-150: 453: 26.75
+	Hs
		1183116 MARK2_HUMAN (2-753): 0: 626: 59.90		NUAK2_HUMAN (1-628): 0: 592: 100.00
+	Hs
		647828 MARK3_HUMAN (1-753): 0: 769: 100.00		MARK3_HUMAN (5-625): 3.05e-141: 430: 25.54
+	Hs
		647947 MARK3_HUMAN (1-753): 0: 769: 100.00		MARK4_HUMAN (17-611): 2.32e-138: 422: 27.19
+	Rn
		139680 MARK2_RAT (2-753): 0: 647: 63.41		MARK2_RAT (6-625): 3.48e-145: 439: 25.60
+	Hs
		1183058 MARK2_HUMAN (2-753): 0: 626: 59.90		MARK2_HUMAN (6-613): 9.9e-150: 453: 26.75
+	Hs
		1183138 MARK1_HUMAN (1-753): 0: 619: 62.89		MARK2_HUMAN (6-613): 9.9e-150: 453: 26.75
+	Hs
		1183095 MARK2_HUMAN (2-753): 0: 626: 59.90		MARK4_HUMAN (17-611): 2.32e-138: 422: 27.19
+	Hs
		647883 MARK3_HUMAN (1-753): 0: 769: 100.00		AAPK1_HUMAN (35-302): 1.31e-70: 240: 46.49
+	Hs
		258840 MARK2_HUMAN (2-753): 0: 626: 59.90		O75271_HUMAN (53-318): 3.6e-74: 246: 46.31
+	At
		1105318 KIN10_ARATH (55-462): 2.55e-150: 449: 34.66		KIN10_ARATH (52-405): 1.01e-140: 421: 36.70
+	Hs
		1025158 MARK2_HUMAN (2-753): 0: 626: 59.90		AAPK1_HUMAN (35-302): 1.31e-70: 240: 46.49
+	At
		955190 KIN10_ARATH (55-462): 2.55e-150: 449: 34.66		KIN11_ARATH (52-405): 7.97e-138: 413: 38.20
+	At
		187503 KIN10_ARATH (55-462): 2.55e-150: 449: 34.66		CIPKB_ARATH (34-400): 1.11e-138: 412: 31.57
+	Hs
		1025228 MARK1_HUMAN (1-753): 0: 619: 62.89		AAPK1_HUMAN (35-302): 1.31e-70: 240: 46.49
+	At
		187501 KIN11_ARATH (55-446): 1.28e-148: 445: 36.68		CIPKB_ARATH (34-400): 1.11e-138: 412: 31.57
+	Hs
		1025197 MARK4_HUMAN (1-753): 0: 612: 60.24		AAPK1_HUMAN (35-302): 1.31e-70: 240: 46.49