HitPredict

Interactor 1: P85A (P27986)
Phosphatidylinositol 3-kinase regulatory subunit alpha (PI3-kinase regulatory subunit alpha) (PI3K regulatory subunit alpha) (PtdIns-3-kinase regulatory subunit alpha) (Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha) (PI3-kinase subunit p85-alpha) (PtdIns-3-kinase regulatory subunit p85-alpha)
Gene: PIK3R1

Interactor 2: BLK (P51451)
Tyrosine-protein kinase Blk (EC 2.7.10.2) (B lymphocyte kinase) (p55-Blk)
Gene: BLK

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PI3K_P85_iSH2~SH2, PK_Tyr_Ser-Thr~SH2, SH2~SH3_1
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 724 Hs			1 505
+	Mm
		557349 P85A_MOUSE (1-724): 0: 592: 95.99		BLK_MOUSE (1-505): 0: 580: 85.74
+	Hs
		442782 P85A_HUMAN (1-724): 0: 596: 100.00		HCK_HUMAN (1-505): 0: 574: 62.92
+	Mm
		411918 P85A_MOUSE (1-724): 0: 592: 95.99		LCK_MOUSE (1-505): 0: 577: 59.35
+	Hs
		411607 P85A_HUMAN (1-724): 0: 596: 100.00		LCK_HUMAN (1-505): 0: 569: 59.15
+	Hs
		412033 P85A_HUMAN (1-724): 0: 596: 100.00		FYN_HUMAN (1-503): 0: 562: 54.75
+	Hs
		522796 P85A_HUMAN (1-724): 0: 596: 100.00		SRC_HUMAN (1-503): 0: 560: 54.22
+	Mm
		643066 P85A_MOUSE (1-724): 0: 592: 95.99		FYN_MOUSE (1-503): 0: 561: 54.56
+	Hs
		433263 P85A_HUMAN (1-724): 0: 596: 100.00		YES_HUMAN (1-503): 0: 555: 53.15
+	Hs
		433595 P85A_HUMAN (1-724): 0: 596: 100.00		LYN_HUMAN (1-505): 0: 551: 59.77
+	Mm
		639435 P85A_MOUSE (1-724): 0: 592: 95.99		LYN_MOUSE (1-505): 0: 554: 60.16
+	Hs
		655254 P85A_HUMAN (1-724): 0: 596: 100.00		ABL2_HUMAN (8-503): 2.23e-165: 500: 38.67
+	Hs
		337356 P85A_HUMAN (1-724): 0: 596: 100.00		ABL1_HUMAN (54-504): 6.77e-163: 492: 41.09
+	Hs
		69202 P85A_HUMAN (1-724): 0: 596: 100.00		A9UF07_HUMAN (54-504): 8.4e-157: 490: 41.09
+	Hs
		655253 P85A_HUMAN (1-724): 0: 596: 100.00		TXK_HUMAN (7-495): 2.05e-160: 467: 34.65
+	Hs
		655339 P85A_HUMAN (1-724): 0: 596: 100.00		ITK_HUMAN (29-496): 7.21e-159: 466: 36.18
+	Mm
		643064 P85A_MOUSE (1-724): 0: 592: 95.99		BTK_MOUSE (4-498): 1.45e-157: 464: 33.73
+	Hs
		129163 P85B_HUMAN (4-719): 6.38e-162: 486: 57.24		LCK_HUMAN (1-505): 0: 569: 59.15
+	Hs
		129164 P85B_HUMAN (4-719): 6.38e-162: 486: 57.24		FYN_HUMAN (1-503): 0: 562: 54.75
+	Hs
		129151 P85B_HUMAN (4-719): 6.38e-162: 486: 57.24		ABL1_HUMAN (54-504): 6.77e-163: 492: 41.09
+	Hs
		69198 P85B_HUMAN (4-719): 6.38e-162: 486: 57.24		A9UF07_HUMAN (54-504): 8.4e-157: 490: 41.09