HitPredict

Interactor 1: BRAF (P28028)
Serine/threonine-protein kinase B-raf (EC 2.7.11.1) (Proto-oncogene B-Raf)
Gene: BRAF

Interactor 2: 1433F (P68510)
14-3-3 protein eta
Gene: Ywhah

Species: Mus musculus

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.775

Property	Details
Experiments	27034005, 8668348
Identification Methods	affinity chromatography technology, two hybrid
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	14-3-3~C1_1, 14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0098793 - presynapse
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 751 Mm			1 246
+	Mm
		655938 BRAF_MOUSE (31-751): 0: 699: 100.00		1433E_MOUSE (1-244): 1.98e-141: 398: 62.71
+	Mm
		655936 BRAF_MOUSE (31-751): 0: 699: 100.00		1433G_MOUSE (1-246): 1.71e-137: 387: 86.99
+	Hs
		542849 BRAF_HUMAN (31-751): 0: 676: 90.90		1433E_HUMAN (1-244): 1.98e-141: 398: 62.71
+	Hs
		110869 H7C560_HUMAN (31-750): 0: 668: 90.62		1433E_HUMAN (1-244): 1.98e-141: 398: 62.71
+	Hs
		542847 BRAF_HUMAN (31-751): 0: 676: 90.90		1433G_HUMAN (1-246): 1.71e-137: 387: 86.99
+	Hs
		542868 BRAF_HUMAN (31-751): 0: 676: 90.90		1433F_HUMAN (1-246): 2.29e-135: 382: 99.19
+	Gg
		982833 BRAF_CHICK (37-751): 0: 657: 89.99		1433G_CHICK (1-246): 5.02e-138: 389: 86.59
+	Gg
		982837 BRAF_CHICK (37-751): 0: 657: 89.99		Q5ZKJ2_CHICK (1-246): 2.8e-135: 382: 98.78
+	Mm
		655943 BRAF_MOUSE (31-751): 0: 699: 100.00		1433Z_MOUSE (3-245): 5.12e-108: 313: 74.07
+	Mm
		655981 BRAF_MOUSE (31-751): 0: 699: 100.00		1433B_MOUSE (3-245): 4.83e-107: 310: 75.92
+	Mm
		655946 BRAF_MOUSE (31-751): 0: 699: 100.00		1433T_MOUSE (3-245): 2.57e-106: 308: 71.84
+	Hs
		542827 BRAF_HUMAN (31-751): 0: 676: 90.90		1433B_HUMAN (3-245): 8.67e-108: 312: 75.51
+	Hs
		542857 BRAF_HUMAN (31-751): 0: 676: 90.90		1433Z_HUMAN (3-245): 1.16e-107: 312: 74.07
+	Hs
		542820 BRAF_HUMAN (31-751): 0: 676: 90.90		1433T_HUMAN (3-245): 4.64e-106: 308: 71.43
+	Hs
		110870 H7C560_HUMAN (31-750): 0: 668: 90.62		1433Z_HUMAN (3-245): 1.16e-107: 312: 74.07
+	Mm
		250867 BRAF_MOUSE (31-751): 0: 699: 100.00		1433S_MOUSE (3-236): 3.13e-94: 278: 65.39
+	Gg
		982836 BRAF_CHICK (37-751): 0: 657: 89.99		1433Z_CHICK (3-245): 2.18e-108: 314: 74.90
+	Gg
		982838 BRAF_CHICK (37-751): 0: 657: 89.99		1433B_CHICK (3-245): 5.99e-107: 310: 75.51
+	Gg
		982840 BRAF_CHICK (37-751): 0: 657: 89.99		1433T_CHICK (3-245): 6.92e-107: 310: 71.84
+	Hs
		542828 BRAF_HUMAN (31-751): 0: 676: 90.90		1433S_HUMAN (3-245): 1.71e-96: 284: 64.20