HitPredict

Interactor 1: EPHB2 (P29323)
Ephrin type-B receptor 2 (EC 2.7.10.1) (Developmentally-regulated Eph-related tyrosine kinase) (ELK-related tyrosine kinase) (EPH tyrosine kinase 3) (EPH-like kinase 5) (EK5) (hEK5) (Renal carcinoma antigen NY-REN-47) (Tyrosine-protein kinase TYRO5) (Tyrosine-protein kinase receptor EPH-3) [Cleaved into: EphB2/CTF1; EphB2/CTF2]
Gene: EPHB2

Interactor 2: EFNB2 (P52799)
Ephrin-B2 (EPH-related receptor tyrosine kinase ligand 5) (LERK-5) (HTK ligand) (HTK-L)
Gene: EFNB2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.85	1.00	0.926

Property	Details
Experiments	26186194, 28514442, 33961781, 11780069, 35384245
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Ephrin~Ephrin_lbd
Common GO terms	GO:0005886 - plasma membrane GO:0007411 - axon guidance GO:0042734 - presynaptic membrane GO:0048013 - ephrin receptor signaling pathway GO:0098978 - glutamatergic synapse
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1055 Hs			1 333
+	Hs
		665579 EPHB2_HUMAN (20-1055): 0: 1304: 100.00		EFNB1_HUMAN (9-333): 2.72e-167: 471: 54.23
+	Mm
		856589 EPHB2_MOUSE (1-985): 0: 1254: 99.49		EFNB2_MOUSE (4-333): 0: 510: 96.97
+	Rn
		454786 EPHB1_RAT (13-985): 0: 1218: 73.79		Q6P7B6_RAT (9-333): 3.46e-165: 465: 54.23
+	Mm
		856298 EPHB1_MOUSE (13-985): 0: 1218: 73.69		EFNB1_MOUSE (9-333): 6.5e-164: 462: 54.23
+	Mm
		856591 EPHA4_MOUSE (6-982): 0: 1155: 58.22		EFNB2_MOUSE (4-333): 0: 510: 96.97
+	Dr
		251124 Q5ZEW1_DANRE (9-983): 0: 1185: 57.93		EFNB2_DANRE (6-333): 2.04e-158: 448: 65.56
+	Hs
		957840 EPHA7_HUMAN (6-982): 0: 1154: 57.46		EFNB1_HUMAN (9-333): 2.72e-167: 471: 54.23
+	Dr
		1140993 Q5ZEW1_DANRE (9-983): 0: 1185: 57.93		Q90Z31_DANRE (15-333): 4.12e-140: 401: 47.88
+	Hs
		595976 EPHB2_HUMAN (20-1055): 0: 1304: 100.00		EFNA1_HUMAN (10-204): 7.81e-74: 228: 22.50
+	Mm
		139052 EPHB2_MOUSE (1-985): 0: 1254: 99.49		EFNA5_MOUSE (15-204): 1.15e-55: 182: 23.65
+	Hs
		856596 EPHB1_HUMAN (20-985): 0: 1211: 73.60		EFNA5_HUMAN (15-204): 1.2e-55: 182: 23.15
+	Dr
		1140992 Q5ZEW1_DANRE (9-983): 0: 1185: 57.93		Q90YC5_DANRE (30-219): 8.7e-64: 203: 25.91
+	Dr
		47532 Q5ZEW1_DANRE (9-983): 0: 1185: 57.93		A0A8M1Q1X6_DANRE (12-219): 1.14e-58: 189: 23.15
+	Dr
		945415 Q5ZEW1_DANRE (9-983): 0: 1185: 57.93		EFNA2_DANRE (28-177): 4.97e-58: 187: 27.27
+	Hs
		856319 EPHA7_HUMAN (6-982): 0: 1154: 57.46		EFNA3_HUMAN (22-239): 4.21e-69: 217: 26.52
+	Dr
		945416 Q5ZEW1_DANRE (9-983): 0: 1185: 57.93		EFNA5_DANRE (14-166): 4.19e-55: 181: 26.83
+	Hs
		856385 EPHA7_HUMAN (6-982): 0: 1154: 57.46		EFNA4_HUMAN (12-198): 5.39e-58: 187: 24.34
+	Hs
		856606 EPHA7_HUMAN (6-982): 0: 1154: 57.46		EFNA5_HUMAN (15-204): 1.2e-55: 182: 23.15
+	Mm
		139053 EPHA7_MOUSE (6-982): 0: 1152: 57.16		EFNA5_MOUSE (15-204): 1.15e-55: 182: 23.65
+	Hs
		217642 EPHA7_HUMAN (6-982): 0: 1154: 57.46		EFNA2_HUMAN (23-160): 7.98e-51: 169: 27.03