HitPredict

Interactor 1: EPHB2 (P29323)
Ephrin type-B receptor 2 (EC 2.7.10.1) (Developmentally-regulated Eph-related tyrosine kinase) (ELK-related tyrosine kinase) (EPH tyrosine kinase 3) (EPH-like kinase 5) (EK5) (hEK5) (Renal carcinoma antigen NY-REN-47) (Tyrosine-protein kinase TYRO5) (Tyrosine-protein kinase receptor EPH-3) [Cleaved into: EphB2/CTF1; EphB2/CTF2]
Gene: EPHB2

Interactor 2: AFAD (P55196)
Afadin (ALL1-fused gene from chromosome 6 protein) (Protein AF-6) (Afadin adherens junction formation factor)
Gene: AFDN

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.43	1.00	0.656

Property	Details
Experiments	9707552
Identification Methods	two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PDZ~fn3, PDZ~SAM_1
Common GO terms	GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0010628 - positive regulation of gene expression
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1055 Hs			1 1824
+	Hs
		876391 EPHA7_HUMAN (6-982): 0: 1154: 57.46		AFAD_HUMAN (1-1824): 0: 1279: 100.00
+	Hs
		872402 EPHB3_HUMAN (17-985): 0: 1142: 70.58		AFAD_HUMAN (1-1824): 0: 1279: 100.00
+	Hs
		665186 EPHA2_HUMAN (18-980): 0: 1041: 47.98		AFAD_HUMAN (1-1824): 0: 1279: 100.00
+	Hs
		171297 EPHB6_HUMAN (20-985): 0: 927: 46.28		AFAD_HUMAN (1-1824): 0: 1279: 100.00
+	Hs
		665663 EPHB2_HUMAN (20-1055): 0: 1304: 100.00		GOPC_HUMAN (744-1099): 5.55e-40: 157: 14.72
+	Hs
		665600 EPHB2_HUMAN (20-1055): 0: 1304: 100.00		SCRIB_HUMAN (989-1133): 4.27e-22: 106: 26.85
+	Hs
		665589 EPHB2_HUMAN (20-1055): 0: 1304: 100.00		PTN13_HUMAN (978-1122): 1.17e-17: 92.2: 29.14
+	Hs
		1072604 EPHA7_HUMAN (6-982): 0: 1154: 57.46		GOPC_HUMAN (744-1099): 5.55e-40: 157: 14.72
+	Hs
		665433 EPHA3_HUMAN (16-980): 0: 1152: 56.37		GOPC_HUMAN (744-1099): 5.55e-40: 157: 14.72
+	Hs
		872713 EPHA4_HUMAN (6-982): 0: 1152: 58.33		GOPC_HUMAN (744-1099): 5.55e-40: 157: 14.72
+	Hs
		872493 EPHA5_HUMAN (6-984): 0: 1148: 56.69		GOPC_HUMAN (744-1099): 5.55e-40: 157: 14.72
+	Hs
		1049958 EPHA7_HUMAN (6-982): 0: 1154: 57.46		SCRIB_HUMAN (989-1133): 4.27e-22: 106: 26.85
+	Hs
		1020163 EPHA7_HUMAN (6-982): 0: 1154: 57.46		PTN13_HUMAN (978-1122): 1.17e-17: 92.2: 29.14
+	Hs
		1072546 EPHA7_HUMAN (6-982): 0: 1154: 57.46		LNX2_HUMAN (908-1089): 8.99e-18: 91.8: 18.13
+	Hs
		665393 EPHA3_HUMAN (16-980): 0: 1152: 56.37		PTN13_HUMAN (978-1122): 1.17e-17: 92.2: 29.14
+	Hs
		872638 EPHA4_HUMAN (6-982): 0: 1152: 58.33		PTN13_HUMAN (978-1122): 1.17e-17: 92.2: 29.14
+	Hs
		872431 EPHA5_HUMAN (6-984): 0: 1148: 56.69		PTN13_HUMAN (978-1122): 1.17e-17: 92.2: 29.14
+	Hs
		600576 EPHA1_HUMAN (12-976): 0: 961: 41.21		KIF14_HUMAN (179-624): 8.03e-59: 227: 18.67
+	Hs
		665228 EPHA2_HUMAN (18-980): 0: 1041: 47.98		PTN13_HUMAN (978-1122): 1.17e-17: 92.2: 29.14
+	Hs
		600564 EPHA1_HUMAN (12-976): 0: 961: 41.21		PTN13_HUMAN (978-1122): 1.17e-17: 92.2: 29.14