HitPredict

Interactor 1: MED16 (P32259)
Mediator of RNA polymerase II transcription subunit 16 (Global transcriptional regulator SIN4) (Mediator complex subunit 16) (SNF1 suppressor protein 4) (SWI4 suppressor protein 5) (Transcriptional silencing factor 3) (YGP1 expression regulatory protein 1)
Gene: SIN4

Interactor 2: MED5 (P53114)
Mediator of RNA polymerase II transcription subunit 5 (Mediator complex subunit 5) (Negative regulator of URS2 protein 1)
Gene: NUT1

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.89	0.90	0.896

Property	Details
Experiments	16429126, 15477388, 26240385, 16554755, 37968396, 24882805
Identification Methods	affinity chromatography technology, two hybrid, tandem affinity purification, pull down
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Med16~Med5
Common GO terms	GO:0005634 - nucleus GO:0016592 - mediator complex GO:0032968 - positive regulation of transcription elongation by RNA polymerase II GO:0051123 - RNA polymerase II preinitiation complex assembly GO:0060261 - positive regulation of transcription initiation by RNA polymerase II and 1 others more... GO:0070847 - core mediator complex
Homologous interactions	0