HitPredict

Interactor 1: PRP19 (P32523)
Pre-mRNA-processing factor 19 (EC 2.3.2.27) (RING-type E3 ubiquitin transferase PRP19)
Gene: PRP19

Interactor 2: CLF1 (Q12309)
Pre-mRNA-splicing factor CLF1 (Crooked neck-like factor 1) (PRP19-associated complex protein 77) (Synthetic lethal with CDC40 protein 3)
Gene: CLF1

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	0.60	0.762

Property	Details
Experiments	11842115, 11805837, 11805826, 11884590, 11804584, 16429126, 16357217, 17515604, 19617314, 37968396, 19704000, 20956557, 14759368, 12509417
Identification Methods	coimmunoprecipitation, anti tag coimmunoprecipitation, tandem affinity purification, affinity chromatography technology, two hybrid, biochemical
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000398 - mRNA splicing, via spliceosome GO:0000974 - Prp19 complex GO:0071006 - U2-type catalytic step 1 spliceosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 503 Sc			1 687
+	Sp
		144108 PRP19_SCHPO (1-480): 1.74e-82: 265: 20.04		CLF1_SCHPO (5-686): 5.61e-121: 377: 39.21
+	Hs
		1318164 PRP19_HUMAN (284-502): 2.22e-40: 153: 9.78		CRNL1_HUMAN (5-685): 4.45e-115: 367: 36.61
+	Sc
		692352 PRP19_YEAST (1-503): 7.56e-93: 293: 100.00		SYF1_YEAST (54-674): 1.42e-57: 211: 11.97
+	Sp
		144131 PRP19_SCHPO (1-480): 1.74e-82: 265: 20.04		SYF1_SCHPO (13-674): 9.29e-65: 230: 13.74
+	Sp
		144093 PRP19_SCHPO (1-480): 1.74e-82: 265: 20.04		PRP39_SCHPO (267-653): 3.13e-23: 106: 14.52
+	Sc
		586378 PRP19_YEAST (1-503): 7.56e-93: 293: 100.00		PRP6_YEAST (28-198): 6.71e-10: 64.6: 13.30
+	Dm
		54163 Q7KLW9_DROME (151-317): 7.02e-15: 78.8: 6.47		SYF1_DROME (3-674): 1.7e-77: 267: 14.23
+	Hs
		174768 WDR5_HUMAN (262-502): 3.82e-53: 184: 13.64		OGT1_HUMAN (3-340): 4.64e-40: 160: 14.12
+	Sp
		1015545 YIDC_SCHPO (87-478): 2.49e-64: 217: 12.72		PRP1_SCHPO (1-392): 2.83e-20: 98.1: 13.22
+	Hs
		1154526 FBW1A_HUMAN (330-502): 2.45e-27: 117: 7.51		NAA16_HUMAN (27-675): 3.7e-53: 198: 10.36
+	Hs
		175300 PRP19_HUMAN (284-502): 2.22e-40: 153: 9.78		OGT1_HUMAN (3-340): 4.64e-40: 160: 14.12
+	Hs
		175317 FBW1A_HUMAN (330-502): 2.45e-27: 117: 7.51		OGT1_HUMAN (3-340): 4.64e-40: 160: 14.12
+	Hs
		903230 WDR5_HUMAN (262-502): 3.82e-53: 184: 13.64		RRP5_HUMAN (376-677): 7.52e-17: 87.3: 13.58
+	Hs
		1336498 PRP19_HUMAN (284-502): 2.22e-40: 153: 9.78		SYF1_HUMAN (2-495): 4.59e-26: 116: 14.67
+	Hs
		903236 WDR5_HUMAN (262-502): 3.82e-53: 184: 13.64		SART3_HUMAN (21-387): 2.31e-11: 69.2: 15.08
+	Hs
		300095 WDR5_HUMAN (262-502): 3.82e-53: 184: 13.64		PRP6_HUMAN (449-672): 5e-10: 64.9: 11.89
+	Hs
		197022 PRP4_HUMAN (293-502): 1.86e-39: 151: 11.87		SART3_HUMAN (21-387): 2.31e-11: 69.2: 15.08
+	Hs
		196929 PRP4_HUMAN (293-502): 1.86e-39: 151: 11.87		PRP6_HUMAN (449-672): 5e-10: 64.9: 11.89
+	Hs
		1199241 FBXW7_HUMAN (198-415): 3.45e-17: 86.8: 13.42		PRP39_HUMAN (187-617): 5.38e-18: 90.3: 13.82
+	Hs
		300222 FBXW7_HUMAN (198-415): 3.45e-17: 86.8: 13.42		PRP6_HUMAN (449-672): 5e-10: 64.9: 11.89