HitPredict

Interactor 1: VPP1 (P32563)
V-type proton ATPase subunit a, vacuolar isoform (V-ATPase a 1 subunit) (V-ATPase 95 kDa subunit) (Vacuolar pH protein 1) (Vacuolar proton pump a subunit) (Vacuolar proton translocating ATPase subunit a 1)
Gene: VPH1

Interactor 2: VATF (P39111)
V-type proton ATPase subunit F (V-ATPase subunit F) (V-ATPase 14 kDa subunit) (Vacuolar proton pump subunit F)
Gene: VMA7

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.90	0.60	0.738

Property	Details
Experiments	11592965, 19081055, 11805826, 16429126, 37968396, 18467557
Identification Methods	coimmunoprecipitation, anti tag coimmunoprecipitation, tandem affinity purification, affinity chromatography technology, protein complementation assay, dihydrofolate reductase reconstruction
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000329 - fungal-type vacuole membrane GO:0007035 - vacuolar acidification GO:0016471 - vacuolar proton-transporting V-type ATPase complex GO:0046961 - proton-transporting ATPase activity, rotational mechanism GO:1902600 - proton transmembrane transport
Homologous interactions	6

Homologous interactions

Color coding for hit scores

	1 840 Sc			1 118
+	Hs
		1088386 VPP1_HUMAN (8-824): 0: 1220: 40.21		VATF_HUMAN (1-116): 7.13e-56: 171: 53.45
+	Hs
		1088398 VPP4_HUMAN (7-824): 0: 1198: 36.82		VATF_HUMAN (1-116): 7.13e-56: 171: 53.45
+	Hs
		1088410 VPP2_HUMAN (7-822): 0: 1152: 36.59		VATF_HUMAN (1-116): 7.13e-56: 171: 53.45
+	Dm
		1099212 Q8IML3_DROME (7-833): 0: 1138: 38.54		VATF1_DROME (2-116): 7.01e-56: 171: 51.30
+	Hs
		1035661 VPP3_HUMAN (7-826): 0: 1034: 35.75		VATF_HUMAN (1-116): 7.13e-56: 171: 53.45
+	Sc
		741296 VPP2_YEAST (4-824): 0: 987: 49.10		VATF_YEAST (1-118): 5.85e-60: 181: 100.00