HitPredict

Interactor 1: GCN1 (P33892)
eIF-2-alpha kinase activator GCN1 (General control non-derepressible protein 1) (Translational activator GCN1)
Gene: GCN1

Interactor 2: MAK5 (P38112)
ATP-dependent RNA helicase MAK5 (EC 3.6.4.13) (Maintenance of killer protein 5)
Gene: MAK5

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.57	0.50	0.537

Property	Details
Experiments	11805826, 16429126
Identification Methods	tandem affinity purification, affinity chromatography technology
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	-
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 2672 Sc			1 773
+	Sc
		415937 GCN1_YEAST (1-2672): 0: 1798: 100.00		DED1_YEAST (146-611): 4.99e-117: 367: 25.31
+	Sc
		707848 GCN1_YEAST (1-2672): 0: 1798: 100.00		DBP8_YEAST (178-619): 1.57e-39: 153: 29.33
+	Sc
		715921 UTP20_YEAST (1367-2411): 3.05e-15: 85.2: 11.49		MAK5_YEAST (1-773): 3.79e-163: 493: 100.00
+	Sp
		140997 2AAA_SCHPO (1640-2239): 9.32e-33: 138: 12.92		DED1_SCHPO (143-616): 4.98e-126: 392: 25.10
+	Sc
		416103 NEW1_YEAST (1361-1636): 7.06e-35: 149: 18.95		DED1_YEAST (146-611): 4.99e-117: 367: 25.31
+	Hs
		134112 CAND1_HUMAN (814-1469): 2.62e-09: 65.1: 13.54		DDX3X_HUMAN (28-618): 9.99e-129: 400: 20.92
+	Hs
		1202027 CAND1_HUMAN (814-1469): 2.62e-09: 65.1: 13.54		DDX17_HUMAN (107-668): 5.25e-127: 397: 22.20
+	Hs
		571509 CAND1_HUMAN (814-1469): 2.62e-09: 65.1: 13.54		DDX5_HUMAN (107-672): 6.11e-125: 389: 22.16
+	Sc
		560966 EF3A_YEAST (1321-1710): 6.99e-83: 299: 22.87		DBP8_YEAST (178-619): 1.57e-39: 153: 29.33
+	Sc
		753164 EF3B_YEAST (1321-1674): 3.58e-82: 297: 22.69		DBP8_YEAST (178-619): 1.57e-39: 153: 29.33
+	Hs
		1202136 CAND1_HUMAN (814-1469): 2.62e-09: 65.1: 13.54		DDX4_HUMAN (47-613): 7.66e-118: 373: 19.51
+	Hs
		1202105 CAND1_HUMAN (814-1469): 2.62e-09: 65.1: 13.54		DDX24_HUMAN (242-734): 2.14e-67: 241: 33.13
+	Sc
		753193 NEW1_YEAST (1361-1636): 7.06e-35: 149: 18.95		DBP8_YEAST (178-619): 1.57e-39: 153: 29.33
+	Sc
		715973 UTP20_YEAST (1367-2411): 3.05e-15: 85.2: 11.49		HAS1_YEAST (178-598): 2.55e-41: 160: 31.46
+	Hs
		1202110 CAND1_HUMAN (814-1469): 2.62e-09: 65.1: 13.54		DDX47_HUMAN (182-577): 1.57e-44: 168: 29.72
+	Hs
		1202140 CAND1_HUMAN (814-1469): 2.62e-09: 65.1: 13.54		DDX18_HUMAN (70-598): 3.25e-38: 153: 29.16