HitPredict

Interactor 1: DID4 (P36108)
DOA4-independent degradation protein 4 (ESCRT-III complex subunit VPS2) (Vacuolar protein-sorting-associated protein 2) (Vacuolar protein-targeting protein 14)
Gene: DID4

Interactor 2: VPS4 (P52917)
Vacuolar protein sorting-associated protein 4 (DOA4-independent degradation protein 6) (Protein END13) (Vacuolar protein-targeting protein 10)
Gene: VPS4

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.90	0.60	0.739

Property	Details
Experiments	17928861, 24711499, 25833946, 18266866, 17408385, 18194652
Identification Methods	pull down, x-ray crystallography, classical fluorescence spectroscopy, comigration in non denaturing gel electrophoresis, affinity chromatography technology, unspecified method, far western blotting, two hybrid
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0015031 - protein transport GO:0045053 - protein retention in Golgi apparatus GO:0045324 - late endosome to vacuole transport GO:0070676 - intralumenal vesicle formation
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 232 Sc			1 437
+	At
		1352475 VPS2A_ARATH (3-230): 6.92e-57: 182: 43.86		VPS4_ARATH (4-436): 3.27e-116: 348: 53.46
+	Sc
		732543 VPS24_YEAST (11-229): 1.98e-40: 139: 22.83		VPS4_YEAST (1-437): 9.44e-127: 375: 100.00
+	Hs
		208804 CHM2A_HUMAN (4-231): 4.05e-47: 156: 39.04		VPS4B_HUMAN (2-436): 2.84e-118: 354: 60.50
+	Mm
		804060 CHM2A_MOUSE (4-231): 1.4e-47: 158: 39.04		VPS4B_MOUSE (2-436): 2.92e-117: 351: 60.72
+	Hs
		208869 CHM2A_HUMAN (4-231): 4.05e-47: 156: 39.04		VPS4A_HUMAN (3-435): 7.08e-117: 350: 58.73
+	At
		1013169 VPS2B_ARATH (5-230): 5.98e-46: 154: 33.19		VPS4_ARATH (4-436): 3.27e-116: 348: 53.46
+	Hs
		260164 CHM2B_HUMAN (10-218): 1.66e-42: 144: 27.27		VPS4B_HUMAN (2-436): 2.84e-118: 354: 60.50
+	Hs
		1357378 CHM2B_HUMAN (10-218): 1.66e-42: 144: 27.27		VPS4A_HUMAN (3-435): 7.08e-117: 350: 58.73
+	Dm
		1362012 Q9VBI3_DROME (4-205): 3.75e-44: 150: 45.55		Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43
+	Dm
		1373132 Q9VRJ5_DROME (3-211): 2.24e-44: 149: 25.36		Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43
+	Hs
		1357379 CHMP3_HUMAN (7-229): 1.76e-40: 139: 21.78		VPS4A_HUMAN (3-435): 7.08e-117: 350: 58.73
+	Dm
		1371193 Q9VN02_DROME (10-229): 9.7e-43: 145: 22.27		Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43
+	At
		1352776 CHM1B_ARATH (24-231): 3.13e-36: 128: 22.12		VPS4_ARATH (4-436): 3.27e-116: 348: 53.46
+	Dm
		1248702 Q8SXB1_DROME (17-205): 1.4e-36: 130: 45.50		Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43
+	Hs
		260156 CHM1A_HUMAN (24-229): 5.01e-33: 119: 19.90		VPS4B_HUMAN (2-436): 2.84e-118: 354: 60.50
+	Hs
		260145 CHM1B_HUMAN (25-205): 4.16e-32: 117: 22.10		VPS4B_HUMAN (2-436): 2.84e-118: 354: 60.50
+	Dm
		1279493 Q95SH2_DROME (25-230): 2.74e-35: 126: 22.82		Q9Y162_DROME (1-436): 3.05e-114: 343: 57.43
+	Hs
		1336687 CHM1A_HUMAN (24-229): 5.01e-33: 119: 19.90		VPS4A_HUMAN (3-435): 7.08e-117: 350: 58.73
+	Hs
		208865 CHM2A_HUMAN (4-231): 4.05e-47: 156: 39.04		SPAST_HUMAN (112-367): 1.5e-66: 225: 46.62
+	Hs
		1185555 CHM1B_HUMAN (25-205): 4.16e-32: 117: 22.10		SPAST_HUMAN (112-367): 1.5e-66: 225: 46.62