HitPredict

Interactor 1: MP2K2 (P36507)
Dual specificity mitogen-activated protein kinase kinase 2 (MAP kinase kinase 2) (MAPKK 2) (EC 2.7.12.2) (ERK activator kinase 2) (MAPK/ERK kinase 2) (MEK 2)
Gene: MAP2K2

Interactor 2: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.789

Property	Details
Experiments	31871319, 32707033
Identification Methods	affinity chromatography technology, pull down
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Pkinase~Ras
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0005925 - focal adhesion GO:0009898 - cytoplasmic side of plasma membrane GO:0071902 - positive regulation of protein serine/threonine kinase activity
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 400 Hs			1 193
+	Hs
		900394 MP2K1_HUMAN (2-399): 2.32e-128: 376: 78.14		RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00
+	Hs
		925211 MP2K1_HUMAN (2-399): 2.32e-128: 376: 78.14		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		734906 MP2K2_HUMAN (1-400): 1.09e-125: 370: 100.00		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		681775 AKT1_HUMAN (43-351): 3.37e-124: 369: 20.12		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		734900 MP2K2_HUMAN (1-400): 1.09e-125: 370: 100.00		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33
+	Hs
		625333 CDK2_HUMAN (69-375): 3.95e-124: 362: 25.16		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		568611 KAPCA_HUMAN (32-347): 6.67e-122: 358: 22.99		RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00
+	Hs
		915619 MP2K1_HUMAN (2-399): 2.32e-128: 376: 78.14		RHOB_HUMAN (1-191): 1.62e-73: 221: 83.94
+	Hs
		436162 CDK2_HUMAN (69-375): 3.95e-124: 362: 25.16		RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71
+	Hs
		568619 KAPCA_HUMAN (32-347): 6.67e-122: 358: 22.99		RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22
+	Hs
		436148 KAPCA_HUMAN (32-347): 6.67e-122: 358: 22.99		RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71
+	Hs
		623871 KPCL_HUMAN (28-354): 2.59e-121: 368: 21.49		RHOB_HUMAN (1-191): 1.62e-73: 221: 83.94
+	Hs
		120398 MP2K1_HUMAN (2-399): 2.32e-128: 376: 78.14		RHOD_HUMAN (7-182): 1.68e-69: 212: 53.98
+	Hs
		568605 KAPCA_HUMAN (32-347): 6.67e-122: 358: 22.99		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33
+	Hs
		545209 KAPCB_HUMAN (62-368): 2.15e-122: 359: 21.54		RAC2_HUMAN (1-193): 3.9e-76: 228: 53.61
+	Hs
		120328 MP2K2_HUMAN (1-400): 1.09e-125: 370: 100.00		RHOD_HUMAN (7-182): 1.68e-69: 212: 53.98
+	Ce
		104466 Q304D8_CAEEL (48-377): 9.06e-104: 314: 29.94		G5EBV3_CAEEL (2-193): 1.08e-77: 232: 50.52
+	Ce
		104472 CST1_CAEEL (48-377): 2.22e-102: 314: 29.94		G5EBV3_CAEEL (2-193): 1.08e-77: 232: 50.52
+	Hs
		805371 MP2K3_HUMAN (15-388): 8.33e-102: 307: 32.47		CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33