HitPredict

Interactor 1: RSP5 (P39940)
E3 ubiquitin-protein ligase RSP5 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase RSP5) (Reverses SPT-phenotype protein 5)
Gene: RSP5

Interactor 2: ALY2 (P47029)
Arrestin-related trafficking adapter 3 (Arrestin-like protein 2)
Gene: ALY2

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	0.60	0.752

Property	Details
Experiments	26508657, 17551511, 18976803, 20489023, 20956561, 16606443, 16554755, 23824189, 16429126
Identification Methods	affinity chromatography technology, enzymatic study, pull down, unspecified method, tandem affinity purification, anti tag coimmunoprecipitation, protein array
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005886 - plasma membrane GO:0070086 - ubiquitin-dependent endocytosis GO:2000397 - positive regulation of ubiquitin-dependent endocytosis
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 809 Sc			1 1046
+	Sc
		733048 RSP5_YEAST (1-809): 0: 825: 100.00		ALY1_YEAST (9-981): 0: 721: 39.06
+	Sc
		765366 RSP5_YEAST (1-809): 0: 825: 100.00		ROD1_YEAST (200-853): 6.29e-97: 327: 17.82
+	Sc
		745491 RSP5_YEAST (1-809): 0: 825: 100.00		ECM21_YEAST (330-849): 5.63e-76: 273: 18.03
+	Sc
		765445 RSP5_YEAST (1-809): 0: 825: 100.00		CSR2_YEAST (330-800): 1.21e-63: 237: 13.97
+	Sc
		765337 RSP5_YEAST (1-809): 0: 825: 100.00		ART5_YEAST (55-587): 8e-57: 209: 13.68
+	Sc
		765302 RSP5_YEAST (1-809): 0: 825: 100.00		ROG3_YEAST (284-796): 5.61e-52: 197: 18.37
+	Sp
		1264987 PUB1_SCHPO (4-809): 0: 782: 63.82		ALY1_SCHPO (27-197): 1.41e-33: 138: 29.00
+	Sp
		145423 PUB3_SCHPO (4-809): 0: 733: 54.05		ALY1_SCHPO (27-197): 1.41e-33: 138: 29.00
+	Hs
		1285047 ITCH_HUMAN (1-809): 0: 727: 34.62		ARRD3_HUMAN (255-601): 1.43e-28: 121: 12.74
+	Hs
		1252807 ITCH_HUMAN (1-809): 0: 727: 34.62		ARRD2_HUMAN (248-572): 1.4e-27: 118: 13.03
+	Hs
		1241904 ITCH_HUMAN (1-809): 0: 727: 34.62		ARRD4_HUMAN (278-570): 3.1e-20: 96.8: 12.23
+	Hs
		1294858 ITCH_HUMAN (1-809): 0: 727: 34.62		TXNIP_HUMAN (250-595): 3.18e-18: 90.2: 11.80
+	Hs
		1285070 WWP1_HUMAN (108-809): 0: 690: 40.33		ARRD3_HUMAN (255-601): 1.43e-28: 121: 12.74
+	Hs
		1252814 WWP1_HUMAN (108-809): 0: 690: 40.33		ARRD2_HUMAN (248-572): 1.4e-27: 118: 13.03
+	Hs
		1234600 ITCH_HUMAN (1-809): 0: 727: 34.62		ARRD1_HUMAN (274-670): 1.26e-13: 76.3: 12.32
+	Hs
		1241917 WWP1_HUMAN (108-809): 0: 690: 40.33		ARRD4_HUMAN (278-570): 3.1e-20: 96.8: 12.23
+	Hs
		1327911 WWP1_HUMAN (108-809): 0: 690: 40.33		TXNIP_HUMAN (250-595): 3.18e-18: 90.2: 11.80
+	Hs
		1234609 SMUF1_HUMAN (4-809): 0: 701: 36.64		ARRD1_HUMAN (274-670): 1.26e-13: 76.3: 12.32
+	Hs
		1234607 WWP1_HUMAN (108-809): 0: 690: 40.33		ARRD1_HUMAN (274-670): 1.26e-13: 76.3: 12.32
+	Hs
		125580 WWP2_HUMAN (100-809): 0: 684: 40.81		ARRD1_HUMAN (274-670): 1.26e-13: 76.3: 12.32