HitPredict

Interactor 1: RSP5 (P39940)
E3 ubiquitin-protein ligase RSP5 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase RSP5) (Reverses SPT-phenotype protein 5)
Gene: RSP5

Interactor 2: RUB1 (Q03919)
NEDD8-like protein RUB1 (Related to ubiquitin protein 1) (Ubiquitin-like protein RUB1)
Gene: RUB1

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.41	1.00	0.647

Property	Details
Experiments	16606443
Identification Methods	unspecified method, protein array
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	HECT~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 809 Sc			1 77
+	Sc
		408371 RSP5_YEAST (1-809): 0: 825: 100.00		RS31_YEAST (1-70): 2.28e-30: 105: 50.00
+	Sc
		477293 RSP5_YEAST (1-809): 0: 825: 100.00		UBI4P_YEAST (1-76): 1.13e-16: 73.9: 52.63
+	Sp
		145418 PUB3_SCHPO (4-809): 0: 733: 54.05		RL402_SCHPO (1-76): 2.69e-33: 112: 52.63
+	Sp
		145416 PUB3_SCHPO (4-809): 0: 733: 54.05		RS27A_SCHPO (1-71): 5.46e-30: 104: 50.70
+	Sp
		145417 PUB3_SCHPO (4-809): 0: 733: 54.05		RS27B_SCHPO (1-71): 4.34e-30: 104: 50.70
+	Hs
		475140 ITCH_HUMAN (1-809): 0: 727: 34.62		UBB_HUMAN (1-76): 2.37e-31: 110: 52.63
+	Hs
		1079609 SMUF1_HUMAN (4-809): 0: 701: 36.64		NEDD8_HUMAN (1-76): 1.61e-34: 113: 59.21
+	Hs
		924156 WWP1_HUMAN (108-809): 0: 690: 40.33		RS27A_HUMAN (1-76): 3.48e-37: 123: 52.63
+	Hs
		125347 WWP2_HUMAN (100-809): 0: 684: 40.81		RS27A_HUMAN (1-76): 3.48e-37: 123: 52.63
+	Hs
		125112 WWP2_HUMAN (100-809): 0: 684: 40.81		UBB_HUMAN (1-76): 2.37e-31: 110: 52.63
+	Hs
		474953 NEDD4_HUMAN (102-807): 0: 668: 39.67		UBB_HUMAN (1-76): 2.37e-31: 110: 52.63
+	Hs
		924414 SMUF2_HUMAN (230-809): 0: 640: 42.86		RL40_HUMAN (1-76): 2.25e-34: 115: 52.63
+	Hs
		1079607 SMUF2_HUMAN (230-809): 0: 640: 42.86		NEDD8_HUMAN (1-76): 1.61e-34: 113: 59.21
+	Hs
		475196 SMUF2_HUMAN (230-809): 0: 640: 42.86		UBB_HUMAN (1-76): 2.37e-31: 110: 52.63
+	Mm
		924239 HUWE1_MOUSE (352-808): 7.81e-167: 538: 37.42		RS27A_MOUSE (1-76): 5.15e-37: 122: 52.63
+	Hs
		924336 HUWE1_HUMAN (252-808): 3.56e-167: 539: 31.78		RL40_HUMAN (1-76): 2.25e-34: 115: 52.63
+	Hs
		475080 HUWE1_HUMAN (252-808): 3.56e-167: 539: 31.78		UBB_HUMAN (1-76): 2.37e-31: 110: 52.63
+	Sc
		477647 TOM1_YEAST (370-808): 5.87e-157: 509: 37.36		RL40A_YEAST (1-76): 8.26e-34: 113: 52.63
+	Sc
		408448 TOM1_YEAST (370-808): 5.87e-157: 509: 37.36		RS31_YEAST (1-70): 2.28e-30: 105: 50.00
+	Sc
		477424 TOM1_YEAST (370-808): 5.87e-157: 509: 37.36		UBI4P_YEAST (1-76): 1.13e-16: 73.9: 52.63