HitPredict

Interactor 1: DOA10 (P40318)
ERAD-associated E3 ubiquitin-protein ligase DOA10 (EC 2.3.2.27) (RING-type E3 ubiquitin transferase DOA10)
Gene: SSM4

Interactor 2: UBC7 (Q02159)
Ubiquitin-conjugating enzyme E2 7 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme 7) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme E2-18 kDa) (Ubiquitin-protein ligase)
Gene: UBC7

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	0.60	0.754

Property	Details
Experiments	27570077, 25646477, 16873066, 33015833, 36325070, 38467638, 21467040, 16179953, 23665229
Identification Methods	enzymatic study, pull down, affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000837 - Doa10p ubiquitin ligase complex GO:0004842 - ubiquitin-protein transferase activity GO:0005783 - endoplasmic reticulum GO:0005789 - endoplasmic reticulum membrane GO:0016567 - protein ubiquitination and 1 others more... GO:0036503 - ERAD pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1319 Sc			1 165
+	Hs
		231862 MARH6_HUMAN (32-163): 5.04e-35: 147: 28.79		UB2D2_HUMAN (4-164): 1.2e-72: 216: 36.03
+	Hs
		849338 MARH8_HUMAN (32-192): 5.21e-38: 146: 17.95		UB2D1_HUMAN (4-164): 7.38e-73: 217: 36.65
+	Hs
		231858 MARH6_HUMAN (32-163): 5.04e-35: 147: 28.79		UB2G2_HUMAN (1-165): 1.05e-71: 214: 62.42
+	Hs
		895806 MARH8_HUMAN (32-192): 5.21e-38: 146: 17.95		UB2D3_HUMAN (4-164): 2.23e-71: 213: 36.03
+	Hs
		231861 MARH6_HUMAN (32-163): 5.04e-35: 147: 28.79		UB2G1_HUMAN (4-162): 7.38e-69: 207: 50.00
+	Hs
		849378 MARH1_HUMAN (8-189): 5.17e-34: 134: 15.74		UB2D1_HUMAN (4-164): 7.38e-73: 217: 36.65
+	Hs
		849469 MARH4_HUMAN (23-177): 9.71e-31: 128: 21.29		UB2D1_HUMAN (4-164): 7.38e-73: 217: 36.65
+	Hs
		849448 MARH7_HUMAN (2-128): 6.58e-27: 120: 22.22		UB2D1_HUMAN (4-164): 7.38e-73: 217: 36.65
+	Hs
		919864 MARH7_HUMAN (2-128): 6.58e-27: 120: 22.22		UB2D2_HUMAN (4-164): 1.2e-72: 216: 36.03
+	Hs
		895915 MARH7_HUMAN (2-128): 6.58e-27: 120: 22.22		UB2D3_HUMAN (4-164): 2.23e-71: 213: 36.03
+	Hs
		1199325 MARH3_HUMAN (26-105): 4.21e-20: 93.2: 26.25		UB2D4_HUMAN (4-164): 1.63e-73: 218: 36.65
+	Hs
		849466 MARH2_HUMAN (31-105): 4.07e-20: 93.2: 29.33		UB2D1_HUMAN (4-164): 7.38e-73: 217: 36.65
+	Hs
		919767 MARH3_HUMAN (26-105): 4.21e-20: 93.2: 26.25		UB2D2_HUMAN (4-164): 1.2e-72: 216: 36.03
+	Hs
		895832 MARH3_HUMAN (26-105): 4.21e-20: 93.2: 26.25		UB2D3_HUMAN (4-164): 2.23e-71: 213: 36.03
+	Hs
		849461 MARH5_HUMAN (39-145): 8.52e-18: 87: 20.87		UB2D1_HUMAN (4-164): 7.38e-73: 217: 36.65
+	Hs
		919880 MARH5_HUMAN (39-145): 8.52e-18: 87: 20.87		UB2D2_HUMAN (4-164): 1.2e-72: 216: 36.03
+	Hs
		895930 MARH5_HUMAN (39-145): 8.52e-18: 87: 20.87		UB2D3_HUMAN (4-164): 2.23e-71: 213: 36.03
+	Hs
		1199317 MARH3_HUMAN (26-105): 4.21e-20: 93.2: 26.25		UB2E2_HUMAN (3-163): 6.79e-68: 206: 34.16
+	Hs
		827980 MARH1_HUMAN (8-189): 5.17e-34: 134: 15.74		UB2R1_HUMAN (4-145): 1.59e-40: 137: 49.30
+	Hs
		828037 MARH2_HUMAN (31-105): 4.07e-20: 93.2: 29.33		UB2R1_HUMAN (4-145): 1.59e-40: 137: 49.30