HitPredict

Interactor 1: MAD2 (P40958)
Mitotic spindle checkpoint component MAD2 (Mitotic MAD2 protein)
Gene: MAD2

Interactor 2: MAD3 (P47074)
Spindle assembly checkpoint component MAD3 (Mitotic MAD3 protein)
Gene: MAD3

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.70	0.60	0.650

Property	Details
Experiments	15879521, 17406666, 10704439
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0007094 - mitotic spindle assembly checkpoint signaling GO:0033597 - mitotic checkpoint complex GO:0044774 - mitotic DNA integrity checkpoint signaling GO:0051301 - cell division GO:1902499 - positive regulation of protein autoubiquitination
Homologous interactions	7

Homologous interactions

Color coding for hit scores

	1 196 Sc			1 515
+	Hs
		236921 MD2L1_HUMAN (3-195): 3.25e-86: 254: 42.78		BUB1B_HUMAN (24-450): 3.12e-122: 385: 23.37
+	Sc
		778482 MAD2_YEAST (1-196): 3.25e-88: 259: 100.00		BUB1_YEAST (45-424): 2.26e-97: 318: 32.55
+	At
		187358 MAD2_ARATH (5-195): 1.86e-86: 255: 42.19		BUBR1_ARATH (25-312): 6.23e-83: 264: 26.23
+	Sp
		145596 MAD2_SCHPO (4-195): 1.74e-81: 242: 48.45		MAD3_SCHPO (13-332): 1.08e-87: 273: 26.30
+	Dm
		51409 Q9VRQ2_DROME (5-195): 3.58e-83: 246: 35.71		A1Z6I7_DROME (25-340): 2.46e-77: 266: 22.74
+	Sp
		145599 MAD2_SCHPO (4-195): 1.74e-81: 242: 48.45		BUB1_SCHPO (23-354): 2e-67: 236: 19.44
+	Hs
		211011 MD2L1_HUMAN (3-195): 3.25e-86: 254: 42.78		BUB1_HUMAN (55-356): 9.98e-49: 182: 17.05