HitPredict

Interactor 1: SCD2 (P40996)
Protein scd2/ral3
Gene: SCD2

Interactor 2: STE20 (P50527)
Serine/threonine-protein kinase shk1/pak1 (EC 2.7.11.1)
Gene: shk1

Species: Schizosaccharomyces pombe 972h-

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.77	0.60	0.682

Property	Details
Experiments	12409291, 10567532, 23615447
Identification Methods	affinity chromatography technology, enzymatic study, far western blotting, two hybrid, protein kinase assay
Interaction Type	physical association, direct interaction, phosphorylation reaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0032153 - cell division site GO:0051286 - cell tip GO:0071521 - Cdc42 GTPase complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 536 Sp			1 658
+	Sc
		666281 BEM1_YEAST (2-492): 2.43e-81: 265: 29.28		STE20_YEAST (12-651): 1.34e-134: 419: 37.39
+	Sc
		666298 BEM1_YEAST (2-492): 2.43e-81: 265: 29.28		SKM1_YEAST (54-647): 2.91e-128: 394: 31.91
+	Hs
		209044 NCK2_HUMAN (4-271): 1.15e-35: 139: 13.41		PAK1_HUMAN (84-658): 4.85e-151: 449: 40.97
+	Hs
		557676 NCK1_HUMAN (3-271): 7.74e-36: 139: 14.80		PAK1_HUMAN (84-658): 4.85e-151: 449: 40.97
+	Mm
		292046 NCK1_MOUSE (3-271): 1.26e-35: 138: 14.80		PAK1_MOUSE (84-658): 2.3e-149: 445: 40.80
+	Hs
		277595 NCK1_HUMAN (3-271): 7.74e-36: 139: 14.80		PAK3_HUMAN (84-658): 1.55e-144: 433: 39.83
+	Hs
		209045 NCK2_HUMAN (4-271): 1.15e-35: 139: 13.41		PAK2_HUMAN (92-658): 3.82e-144: 431: 39.51
+	Hs
		557677 NCK1_HUMAN (3-271): 7.74e-36: 139: 14.80		PAK2_HUMAN (92-658): 3.82e-144: 431: 39.51
+	Hs
		537638 NCF1_HUMAN (2-296): 8.15e-25: 108: 12.50		PAK1_HUMAN (84-658): 4.85e-151: 449: 40.97
+	Hs
		1026922 SPD2A_HUMAN (299-470): 1.12e-28: 123: 21.93		PAK2_HUMAN (92-658): 3.82e-144: 431: 39.51
+	Hs
		580997 PLCG1_HUMAN (29-174): 1.06e-17: 89: 15.82		PAK1_HUMAN (84-658): 4.85e-151: 449: 40.97
+	Hs
		166571 SPD2A_HUMAN (299-470): 1.12e-28: 123: 21.93		DCLK1_HUMAN (83-645): 1.98e-127: 395: 19.86
+	Hs
		277606 ITSN1_HUMAN (94-470): 3.73e-16: 84.4: 8.87		PAK3_HUMAN (84-658): 1.55e-144: 433: 39.83
+	Hs
		537603 NCF1_HUMAN (2-296): 8.15e-25: 108: 12.50		AKT1_HUMAN (257-656): 1.14e-129: 392: 23.67
+	Hs
		580944 PLCG1_HUMAN (29-174): 1.06e-17: 89: 15.82		AKT1_HUMAN (257-656): 1.14e-129: 392: 23.67
+	Sc
		666263 BEM1_YEAST (2-492): 2.43e-81: 265: 29.28		CLA4_YEAST (54-325): 4.41e-25: 112: 21.48
+	Hs
		557731 NCK1_HUMAN (3-271): 7.74e-36: 139: 14.80		M4K3_HUMAN (380-655): 5.08e-58: 212: 42.25
+	Hs
		557840 NCK1_HUMAN (3-271): 7.74e-36: 139: 14.80		M4K5_HUMAN (380-639): 1.25e-57: 210: 42.11
+	Hs
		1217590 ITSN2_HUMAN (65-456): 6.92e-24: 108: 11.01		M4K3_HUMAN (380-655): 5.08e-58: 212: 42.25
+	Dm
		1214366 NCK_DROME (31-297): 1.05e-27: 118: 16.84		PAK_DROME (77-213): 7.03e-18: 89.7: 26.28