HitPredict

Interactor 1: CSK (P41241)
Tyrosine-protein kinase CSK (EC 2.7.10.2) (C-Src kinase) (Protein-tyrosine kinase MPK-2) (p50CSK)
Gene: CSK

Interactor 2: PHAG1 (Q3U1F9)
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (Csk-binding protein) (Transmembrane phosphoprotein Cbp)
Gene: Pag1

Species: Mus musculus

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.793

Property	Details
Experiments	16920712, 26512138
Identification Methods	two hybrid, affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	PAG~SH2
Common GO terms	GO:0002250 - adaptive immune response GO:0005886 - plasma membrane GO:0035556 - intracellular signal transduction GO:0050863 - regulation of T cell activation
Homologous interactions	11

Homologous interactions

Color coding for hit scores

	1 450 Mm			1 429
+	Rn
		693647 CSK_RAT (1-450): 0: 549: 99.33		PHAG1_RAT (1-429): 0: 764: 89.51
+	Mm
		763390 FYN_MOUSE (12-442): 3.41e-171: 493: 37.22		PHAG1_MOUSE (1-429): 0: 785: 100.00
+	Mm
		411925 LCK_MOUSE (6-443): 8.43e-169: 485: 38.41		PHAG1_MOUSE (1-429): 0: 785: 100.00
+	Hs
		782829 CSK_HUMAN (1-450): 0: 548: 99.11		PHAG1_HUMAN (1-429): 0: 705: 78.21
+	Mm
		639443 LYN_MOUSE (4-445): 1.24e-161: 467: 37.69		PHAG1_MOUSE (1-429): 0: 785: 100.00
+	Hs
		337571 ABL1_HUMAN (5-446): 1.08e-164: 495: 38.14		PHAG1_HUMAN (1-429): 0: 705: 78.21
+	Hs
		412375 FYN_HUMAN (12-442): 2.68e-171: 493: 37.22		PHAG1_HUMAN (1-429): 0: 705: 78.21
+	Hs
		523175 SRC_HUMAN (12-442): 8.06e-171: 491: 39.78		PHAG1_HUMAN (1-429): 0: 705: 78.21
+	Hs
		411873 LCK_HUMAN (6-443): 1.62e-168: 485: 38.85		PHAG1_HUMAN (1-429): 0: 705: 78.21
+	Hs
		789135 FRK_HUMAN (11-442): 1.23e-165: 477: 36.87		PHAG1_HUMAN (1-429): 0: 705: 78.21
+	Hs
		434038 LYN_HUMAN (4-445): 1.69e-161: 467: 37.91		PHAG1_HUMAN (1-429): 0: 705: 78.21