HitPredict

Interactor 1: M3K8 (P41279)
Mitogen-activated protein kinase kinase kinase 8 (EC 2.7.11.25) (Cancer Osaka thyroid oncogene) (Proto-oncogene c-Cot) (Serine/threonine-protein kinase cot) (Tumor progression locus 2) (TPL-2)
Gene: MAP3K8

Interactor 2: TF65 (Q04206)
Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
Gene: RELA

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.82	0.60	0.702

Property	Details
Experiments	25852190, 14743216, 25331947, 29084252
Identification Methods	affinity chromatography technology, fluorescent resonance energy transfer, tandem affinity purification, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol
Homologous interactions	9

Homologous interactions

Color coding for hit scores

	1 467 Hs			1 551
+	Hs
		783319 M3K8_HUMAN (1-467): 9.62e-140: 411: 100.00		REL_HUMAN (12-523): 3.56e-165: 484: 41.64
+	Hs
		682168 AKT2_HUMAN (31-438): 7.59e-137: 404: 15.46		REL_HUMAN (12-523): 3.56e-165: 484: 41.64
+	Mm
		638451 M3K8_MOUSE (1-467): 1.09e-138: 408: 93.36		NFKB1_MOUSE (7-451): 3.1e-157: 475: 30.19
+	Hs
		587460 M3K8_HUMAN (1-467): 9.62e-140: 411: 100.00		NFKB1_HUMAN (7-451): 2.23e-153: 465: 29.51
+	Rn
		812706 AKT1_RAT (43-466): 3.95e-134: 397: 15.64		NFKB1_RAT (7-451): 3.65e-154: 467: 30.06
+	Hs
		783315 M3K8_HUMAN (1-467): 9.62e-140: 411: 100.00		NFKB2_HUMAN (7-409): 7.21e-137: 420: 34.75
+	Hs
		981702 M3K14_HUMAN (107-382): 8.18e-30: 125: 30.66		REL_HUMAN (12-523): 3.56e-165: 484: 41.64
+	Hs
		964521 M3K14_HUMAN (107-382): 8.18e-30: 125: 30.66		RELB_HUMAN (3-461): 2.62e-154: 455: 40.00
+	Hs
		961425 M3K14_HUMAN (107-382): 8.18e-30: 125: 30.66		NFKB2_HUMAN (7-409): 7.21e-137: 420: 34.75