HitPredict

Interactor 1: NEDD4 (P46934)
E3 ubiquitin-protein ligase NEDD4 (EC 2.3.2.26) (Cell proliferation-inducing gene 53 protein) (HECT-type E3 ubiquitin transferase NEDD4) (Neural precursor cell expressed developmentally down-regulated protein 4) (NEDD-4)
Gene: NEDD4

Interactor 2: SMAD1 (Q15797)
Mothers against decapentaplegic homolog 1 (MAD homolog 1) (Mothers against DPP homolog 1) (JV4-1) (Mad-related protein 1) (SMAD family member 1) (SMAD 1) (Smad1) (hSMAD1) (Transforming growth factor-beta-signaling protein 1) (BSP-1)
Gene: SMAD1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	0.60	0.600

Property	Details
Experiments	21308777, 15231748
Identification Methods	affinity chromatography technology, two hybrid
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000785 - chromatin GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0032991 - protein-containing complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1319 Hs			1 465
+	Hs
		808240 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD5_HUMAN (2-465): 0: 619: 89.46
+	Hs
		808087 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD3_HUMAN (3-465): 0: 556: 59.66
+	Hs
		808134 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD2_HUMAN (3-465): 0: 541: 55.14
+	Hs
		206944 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD6_HUMAN (36-443): 2.87e-93: 292: 25.61
+	Hs
		167342 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD7_HUMAN (30-131): 2.89e-25: 109: 32.46
+	Hs
		950249 NED4L_HUMAN (517-1319): 0: 934: 62.26		SMAD3_HUMAN (3-465): 0: 556: 59.66
+	Hs
		1078629 NED4L_HUMAN (517-1319): 0: 934: 62.26		SMAD2_HUMAN (3-465): 0: 541: 55.14
+	Hs
		206971 NED4L_HUMAN (517-1319): 0: 934: 62.26		SMAD6_HUMAN (36-443): 2.87e-93: 292: 25.61
+	Hs
		167385 NED4L_HUMAN (517-1319): 0: 934: 62.26		SMAD7_HUMAN (30-131): 2.89e-25: 109: 32.46
+	Hs
		1078757 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD1_HUMAN (1-465): 0: 658: 100.00
+	Hs
		1305881 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD5_HUMAN (2-465): 0: 619: 89.46
+	Hs
		950278 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD3_HUMAN (3-465): 0: 556: 59.66
+	Mm
		1143948 ITCH_MOUSE (433-653): 3.68e-52: 200: 16.33		SMAD2_MOUSE (3-465): 0: 543: 55.14
+	Hs
		1078654 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD2_HUMAN (3-465): 0: 541: 55.14
+	Hs
		125369 WWP2_HUMAN (461-817): 1.56e-41: 167: 17.91		SMAD3_HUMAN (3-465): 0: 556: 59.66
+	Hs
		1035583 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD4_HUMAN (13-454): 1.6e-150: 441: 34.28
+	Hs
		206975 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD6_HUMAN (36-443): 2.87e-93: 292: 25.61
+	Hs
		125023 WWP2_HUMAN (461-817): 1.56e-41: 167: 17.91		SMAD6_HUMAN (36-443): 2.87e-93: 292: 25.61
+	Hs
		167393 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD7_HUMAN (30-131): 2.89e-25: 109: 32.46
+	Hs
		125003 WWP2_HUMAN (461-817): 1.56e-41: 167: 17.91		SMAD7_HUMAN (30-131): 2.89e-25: 109: 32.46