HitPredict

Interactor 1: NEDD4 (P46934)
E3 ubiquitin-protein ligase NEDD4 (EC 2.3.2.26) (Cell proliferation-inducing gene 53 protein) (HECT-type E3 ubiquitin transferase NEDD4) (Neural precursor cell expressed developmentally down-regulated protein 4) (NEDD-4)
Gene: NEDD4

Interactor 2: SMAD5 (Q99717)
Mothers against decapentaplegic homolog 5 (MAD homolog 5) (Mothers against DPP homolog 5) (JV5-1) (SMAD family member 5) (SMAD 5) (Smad5) (hSmad5)
Gene: SMAD5

Species: Homo sapiens

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.43	0.60	0.508

Property	Details
Experiments	15231748
Identification Methods	two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000785 - chromatin GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0032991 - protein-containing complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1319 Hs			1 465
+	Hs
		808135 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD1_HUMAN (3-465): 0: 637: 89.68
+	Hs
		808087 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD3_HUMAN (4-465): 0: 550: 58.97
+	Hs
		808134 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD2_HUMAN (4-465): 0: 530: 55.60
+	Hs
		206944 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD6_HUMAN (37-191): 4.62e-40: 152: 31.01
+	Hs
		167342 NEDD4_HUMAN (1-1319): 0: 1956: 100.00		SMAD7_HUMAN (31-132): 7.88e-27: 113: 31.58
+	Hs
		950249 NED4L_HUMAN (517-1319): 0: 934: 62.26		SMAD3_HUMAN (4-465): 0: 550: 58.97
+	Hs
		1078629 NED4L_HUMAN (517-1319): 0: 934: 62.26		SMAD2_HUMAN (4-465): 0: 530: 55.60
+	Hs
		206971 NED4L_HUMAN (517-1319): 0: 934: 62.26		SMAD6_HUMAN (37-191): 4.62e-40: 152: 31.01
+	Hs
		167385 NED4L_HUMAN (517-1319): 0: 934: 62.26		SMAD7_HUMAN (31-132): 7.88e-27: 113: 31.58
+	Hs
		1305881 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD5_HUMAN (1-465): 0: 650: 100.00
+	Hs
		1078757 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD1_HUMAN (3-465): 0: 637: 89.68
+	Hs
		950278 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD3_HUMAN (4-465): 0: 550: 58.97
+	Mm
		1143948 ITCH_MOUSE (433-653): 3.68e-52: 200: 16.33		SMAD2_MOUSE (4-465): 0: 532: 55.60
+	Hs
		1078654 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD2_HUMAN (4-465): 0: 530: 55.60
+	Hs
		125369 WWP2_HUMAN (461-817): 1.56e-41: 167: 17.91		SMAD3_HUMAN (4-465): 0: 550: 58.97
+	Hs
		1035583 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD4_HUMAN (14-462): 1.05e-149: 439: 34.26
+	Hs
		206975 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD6_HUMAN (37-191): 4.62e-40: 152: 31.01
+	Hs
		125023 WWP2_HUMAN (461-817): 1.56e-41: 167: 17.91		SMAD6_HUMAN (37-191): 4.62e-40: 152: 31.01
+	Hs
		167393 WWP1_HUMAN (389-800): 7.48e-49: 191: 17.86		SMAD7_HUMAN (31-132): 7.88e-27: 113: 31.58
+	Hs
		125003 WWP2_HUMAN (461-817): 1.56e-41: 167: 17.91		SMAD7_HUMAN (31-132): 7.88e-27: 113: 31.58