HitPredict

Interactor 1: VPS25 (P47142)
Vacuolar protein-sorting-associated protein 25 (ESCRT-II complex subunit VPS25)
Gene: VPS25

Interactor 2: VPS20 (Q04272)
Vacuolar protein sorting-associated protein 20 (Amino acid sensor-independent protein 10) (Charged multivesicular body protein 6) (ESCRT-III complex subunit VPS20) (Vacuolar protein-targeting protein 20)
Gene: VPS20

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.84	0.60	0.714

Property	Details
Experiments	11283351, 15086794, 15469844, 18719252, 19135892
Identification Methods	two hybrid pooling approach, two hybrid, pull down, fluorescence technology
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0043162 - ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:1904669 - ATP export
Homologous interactions	6

Homologous interactions

Color coding for hit scores

	1 202 Sc			1 221
+	At
		1258413 VPS25_ARATH (4-200): 1.78e-65: 201: 26.40		VP202_ARATH (6-216): 5.3e-29: 109: 27.23
+	At
		1209622 VPS25_ARATH (4-200): 1.78e-65: 201: 26.40		VP201_ARATH (6-211): 6.51e-28: 106: 28.37
+	Hs
		1291670 VPS25_HUMAN (5-199): 4.42e-64: 197: 27.69		CHMP6_HUMAN (3-205): 3.76e-26: 101: 26.34
+	Ce
		1308409 Q9U354_CAEEL (5-189): 2.67e-61: 190: 27.03		Q9BL60_CAEEL (3-209): 7.8e-26: 101: 27.75
+	Dm
		91262 VPS25_DROME (5-190): 6.57e-63: 194: 24.19		E4NKK3_DROME (2-207): 1.19e-23: 95.7: 26.44
+	Dm
		1177963 VPS25_DROME (5-190): 6.57e-63: 194: 24.19		Q9W236_DROME (2-207): 4.07e-23: 94.1: 26.44