HitPredict

Interactor 1: UBE2A (P49459)
Ubiquitin-conjugating enzyme E2 A (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme A) (RAD6 homolog A) (HR6A) (hHR6A) (Ubiquitin carrier protein A) (Ubiquitin-protein ligase A)
Gene: UBE2A

Interactor 2: BRE1A (Q5VTR2)
E3 ubiquitin-protein ligase BRE1A (BRE1-A) (hBRE1) (EC 2.3.2.27) (RING finger protein 20) (RING-type E3 ubiquitin transferase BRE1A)
Gene: RNF20

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.90	1.00	0.950

Property	Details
Experiments	25582440, 28031328, 21443952, 19410543, 37633270, 24837678
Identification Methods	affinity chromatography technology, pull down, enzymatic study
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4
Common GO terms	GO:0000209 - protein polyubiquitination GO:0004842 - ubiquitin-protein transferase activity GO:0005654 - nucleoplasm GO:0006511 - ubiquitin-dependent protein catabolic process GO:0031625 - ubiquitin protein ligase binding and 1 others more... GO:0033503 - HULC complex
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 152 Hs			1 975
+	Hs
		919748 UB2D2_HUMAN (5-149): 7.73e-78: 229: 38.62		BRE1A_HUMAN (1-975): 1.48e-77: 275: 100.00
+	Hs
		927757 UBE2B_HUMAN (1-151): 3.28e-74: 219: 96.03		BRE1A_HUMAN (1-975): 1.48e-77: 275: 100.00
+	Hs
		851821 UB2E1_HUMAN (3-150): 5.05e-71: 213: 36.49		BRE1A_HUMAN (1-975): 1.48e-77: 275: 100.00
+	At
		639387 UBC1_ARATH (1-150): 1.31e-73: 218: 76.67		BRE1B_ARATH (45-974): 3.82e-68: 247: 19.98
+	At
		790157 UBC2_ARATH (1-150): 1.51e-73: 218: 76.00		BRE1B_ARATH (45-974): 3.82e-68: 247: 19.98
+	Hs
		256237 UB2D2_HUMAN (5-149): 7.73e-78: 229: 38.62		BRE1B_HUMAN (1-974): 4.61e-62: 230: 56.49
+	Hs
		256227 UBE2A_HUMAN (1-152): 3.88e-76: 225: 100.00		BRE1B_HUMAN (1-974): 4.61e-62: 230: 56.49
+	Hs
		256239 UBE2B_HUMAN (1-151): 3.28e-74: 219: 96.03		BRE1B_HUMAN (1-974): 4.61e-62: 230: 56.49
+	Hs
		256229 UB2E1_HUMAN (3-150): 5.05e-71: 213: 36.49		BRE1B_HUMAN (1-974): 4.61e-62: 230: 56.49
+	Sc
		410240 UBC2_YEAST (1-150): 2.29e-73: 218: 69.33		BRE1_YEAST (509-974): 2.63e-58: 215: 19.14
+	Sp
		251716 UBC2_SCHPO (1-150): 2.83e-70: 209: 72.00		BRL2_SCHPO (320-974): 8.96e-60: 219: 17.80
+	At
		639386 UBC1_ARATH (1-150): 1.31e-73: 218: 76.67		BRE1A_ARATH (149-974): 4.33e-47: 184: 16.91
+	At
		790156 UBC2_ARATH (1-150): 1.51e-73: 218: 76.00		BRE1A_ARATH (149-974): 4.33e-47: 184: 16.91
+	Ce
		712245 UBC2_CAEEL (5-149): 9.01e-78: 228: 38.62		BRE1_CAEEL (1-762): 1.85e-20: 99.6: 17.41
+	Sp
		618356 UBC2_SCHPO (1-150): 2.83e-70: 209: 72.00		BRL1_SCHPO (502-974): 3.59e-26: 117: 16.84
+	Ce
		712246 UBC1_CAEEL (1-149): 2.14e-72: 216: 85.91		BRE1_CAEEL (1-762): 1.85e-20: 99.6: 17.41