HitPredict

Interactor 1: GBG4 (P50150)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4
Gene: GNG4

Interactor 2: GBB1 (P62873)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (Transducin beta chain 1)
Gene: GNB1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.84	1.00	0.921

Property	Details
Experiments	28514442, 8636150, 19168127, 7665596, 12782285
Identification Methods	affinity chromatography technology, two hybrid, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	G-gamma~WD40
Common GO terms	GO:0005834 - heterotrimeric G-protein complex GO:0005886 - plasma membrane GO:0007186 - G protein-coupled receptor signaling pathway GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 75 Hs			1 340
+	Hs
		837673 GBG4_HUMAN (1-75): 1.41e-30: 103: 100.00		GBB2_HUMAN (1-340): 1e-105: 314: 90.29
+	Hs
		921516 GBG3_HUMAN (1-75): 7.91e-30: 102: 69.33		GBB2_HUMAN (1-340): 1e-105: 314: 90.29
+	Hs
		837682 GBG4_HUMAN (1-75): 1.41e-30: 103: 100.00		GBB4_HUMAN (1-340): 6.23e-105: 312: 90.88
+	Hs
		560864 GBG4_HUMAN (1-75): 1.41e-30: 103: 100.00		GBB3_HUMAN (1-340): 1.83e-104: 311: 83.24
+	Hs
		920932 GBG3_HUMAN (1-75): 7.91e-30: 102: 69.33		GBB1_HUMAN (1-340): 7.02e-105: 312: 100.00
+	Hs
		929157 GBG3_HUMAN (1-75): 7.91e-30: 102: 69.33		GBB4_HUMAN (1-340): 6.23e-105: 312: 90.88
+	Hs
		560872 GBG3_HUMAN (1-75): 7.91e-30: 102: 69.33		GBB3_HUMAN (1-340): 1.83e-104: 311: 83.24
+	Hs
		887097 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GBB2_HUMAN (1-340): 1e-105: 314: 90.29
+	Hs
		887096 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GBB1_HUMAN (1-340): 7.02e-105: 312: 100.00
+	Hs
		887126 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GBB4_HUMAN (1-340): 6.23e-105: 312: 90.88
+	Bt
		920925 GBG2_BOVIN (5-75): 1.22e-28: 99: 77.47		GBB1_BOVIN (1-340): 7.02e-105: 312: 100.00
+	Mm
		921088 GBG2_MOUSE (5-75): 1.22e-28: 99: 77.47		GBB1_MOUSE (1-340): 7.02e-105: 312: 100.00
+	Hs
		560868 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GBB3_HUMAN (1-340): 1.83e-104: 311: 83.24
+	Hs
		560895 GBG8_HUMAN (7-75): 3.45e-25: 90.1: 60.87		GBB3_HUMAN (1-340): 1.83e-104: 311: 83.24
+	Mm
		666790 GBG4_MOUSE (1-75): 4.88e-30: 102: 98.67		GBB4_MOUSE (1-340): 3.4e-98: 295: 89.71
+	Hs
		154666 GBG4_HUMAN (1-75): 1.41e-30: 103: 100.00		GNB5_HUMAN (129-340): 1.35e-45: 161: 21.46
+	Hs
		154672 GBG3_HUMAN (1-75): 7.91e-30: 102: 69.33		GNB5_HUMAN (129-340): 1.35e-45: 161: 21.46
+	Mm
		837708 GBG4_MOUSE (1-75): 4.88e-30: 102: 98.67		GNB5_MOUSE (129-340): 1.22e-45: 161: 21.92
+	Hs
		154670 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GNB5_HUMAN (129-340): 1.35e-45: 161: 21.46
+	Hs
		154700 GBG8_HUMAN (7-75): 3.45e-25: 90.1: 60.87		GNB5_HUMAN (129-340): 1.35e-45: 161: 21.46