HitPredict

Interactor 1: UB2D1 (P51668)
Ubiquitin-conjugating enzyme E2 D1 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D1) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D1) (Stimulator of Fe transport) (SFT) (UBC4/5 homolog) (UbcH5) (Ubiquitin carrier protein D1) (Ubiquitin-conjugating enzyme E2(17)KB 1) (Ubiquitin-conjugating enzyme E2-17 kDa 1) (Ubiquitin-protein ligase D1)
Gene: UBE2D1

Interactor 2: RFWD3 (Q6PCD5)
E3 ubiquitin-protein ligase RFWD3 (EC 2.3.2.27) (RING finger and WD repeat domain-containing protein 3) (RING finger protein 201)
Gene: RFWD3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.66	1.00	0.815

Property	Details
Experiments	19690564, 19549727
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_2
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0061630 - ubiquitin protein ligase activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 147 Hs			1 774
+	Hs
		1160856 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RFWD3_HUMAN (1-774): 0: 851: 100.00
+	Zm
		42874 B4FC97_MAIZE (1-147): 1.18e-82: 241: 76.19		A0A1D6P951_MAIZE (286-771): 3.73e-133: 408: 22.39
+	Hs
		1318719 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRIM5_HUMAN (283-645): 1.97e-56: 202: 13.90
+	Hs
		849439 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRIM5_HUMAN (283-645): 1.97e-56: 202: 13.90
+	Hs
		941604 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00
+	Hs
		849306 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00
+	Hs
		1304199 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RING2_HUMAN (234-441): 1.9e-31: 127: 15.39
+	Hs
		849421 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RING2_HUMAN (234-441): 1.9e-31: 127: 15.39
+	Hs
		1313520 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RN126_HUMAN (127-338): 1.78e-29: 121: 16.06
+	Hs
		849428 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RN126_HUMAN (127-338): 1.78e-29: 121: 16.06
+	Zm
		74334 B4FC97_MAIZE (1-147): 1.18e-82: 241: 76.19		B6U926_MAIZE (168-374): 3.49e-27: 116: 16.28
+	Zm
		79637 B6SZE0_MAIZE (1-147): 1.32e-82: 241: 75.51		B6U926_MAIZE (168-374): 3.49e-27: 116: 16.28
+	Hs
		122275 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RN103_HUMAN (205-331): 1.48e-25: 114: 26.06
+	Hs
		584907 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RO52_HUMAN (283-513): 9.24e-27: 116: 13.07
+	Hs
		122256 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RN103_HUMAN (205-331): 1.48e-25: 114: 26.06
+	Hs
		988038 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RING1_HUMAN (238-402): 5.7e-25: 110: 17.58
+	At
		715137 UBC9_ARATH (1-147): 8.78e-82: 238: 77.55		RNG1L_ARATH (172-364): 6.54e-26: 111: 12.18
+	Hs
		849312 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RING1_HUMAN (238-402): 5.7e-25: 110: 17.58
+	Hs
		1290204 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RN125_HUMAN (254-405): 1.16e-23: 102: 24.05
+	Hs
		849405 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RN125_HUMAN (254-405): 1.16e-23: 102: 24.05