HitPredict

Interactor 1: UB2D1 (P51668)
Ubiquitin-conjugating enzyme E2 D1 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D1) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D1) (Stimulator of Fe transport) (SFT) (UBC4/5 homolog) (UbcH5) (Ubiquitin carrier protein D1) (Ubiquitin-conjugating enzyme E2(17)KB 1) (Ubiquitin-conjugating enzyme E2-17 kDa 1) (Ubiquitin-protein ligase D1)
Gene: UBE2D1

Interactor 2: RNF25 (Q96BH1)
E3 ubiquitin-protein ligase RNF25 (EC 2.3.2.27) (RING finger protein 25) (RING finger protein AO7)
Gene: RNF25

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.90	1.00	0.952

Property	Details
Experiments	32296183, 33961781, 19690564, 19549727, 14517261, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, pull down, two hybrid array, two hybrid prey pooling approach, validated two hybrid, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	RWD~UQ_con, UQ_con~zf-RING_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005634 - nucleus GO:0005829 - cytosol GO:0006511 - ubiquitin-dependent protein catabolic process GO:0061630 - ubiquitin protein ligase activity
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 147 Hs			1 459
+	Mm
		919926 UB2D2_MOUSE (1-147): 7.7e-82: 238: 89.12		RNF25_MOUSE (1-459): 0: 669: 82.35
+	Hs
		1285495 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Mm
		854556 UB2E3_MOUSE (4-146): 1.2e-73: 220: 62.94		RNF25_MOUSE (1-459): 0: 669: 82.35
+	Hs
		919815 UB2D2_HUMAN (1-147): 7.7e-82: 238: 89.12		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Hs
		895869 UB2D3_HUMAN (1-147): 9.08e-81: 236: 88.44		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Mm
		854550 UB2E1_MOUSE (4-146): 2.18e-72: 216: 62.94		RNF25_MOUSE (1-459): 0: 669: 82.35
+	Hs
		1285490 UB2E2_HUMAN (4-146): 3.11e-74: 221: 62.94		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Hs
		1282323 UB2E3_HUMAN (4-146): 1.2e-73: 220: 62.94		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Hs
		851863 UB2E1_HUMAN (4-146): 3.94e-72: 216: 62.94		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Dm
		54265 UBCD1_DROME (1-147): 1.87e-81: 238: 89.12		A1Z9I6_DROME (15-257): 1.93e-84: 265: 35.92
+	Dm
		54267 UBCD2_DROME (4-146): 6.98e-74: 221: 64.34		A1Z9I6_DROME (15-257): 1.93e-84: 265: 35.92
+	At
		1118123 UBC29_ARATH (1-147): 1.39e-78: 230: 74.15		Q4TU20_ARATH (23-277): 3.55e-80: 254: 25.09
+	At
		1258961 UBC29_ARATH (1-147): 1.39e-78: 230: 74.15		Q8W4A3_ARATH (128-277): 2.36e-44: 157: 24.16
+	Zm
		41536 B4FC97_MAIZE (1-147): 1.18e-82: 241: 76.19		A0A1D6NML1_MAIZE (33-127): 4.84e-25: 107: 25.00