HitPredict

Interactor 1: UB2D1 (P51668)
Ubiquitin-conjugating enzyme E2 D1 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D1) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D1) (Stimulator of Fe transport) (SFT) (UBC4/5 homolog) (UbcH5) (Ubiquitin carrier protein D1) (Ubiquitin-conjugating enzyme E2(17)KB 1) (Ubiquitin-conjugating enzyme E2-17 kDa 1) (Ubiquitin-protein ligase D1)
Gene: UBE2D1

Interactor 2: TRIM1 (Q9UJV3)
Probable E3 ubiquitin-protein ligase MID2 (EC 2.3.2.27) (Midin-2) (Midline defect 2) (Midline-2) (RING finger protein 60) (RING-type E3 ubiquitin transferase MID2) (Tripartite motif-containing protein 1)
Gene: MID2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.83	1.00	0.912

Property	Details
Experiments	25416956, 32296183, 21143188, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, validated two hybrid, two hybrid prey pooling approach, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 147 Hs			1 735
+	Hs
		1356055 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRIM1_HUMAN (1-735): 0: 592: 100.00
+	Hs
		1151762 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		Q6GX22_HUMAN (21-735): 0: 550: 95.66
+	Hs
		175730 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60
+	Hs
		175653 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60
+	Hs
		1052807 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI25_HUMAN (24-706): 9.41e-99: 319: 20.96
+	Hs
		849330 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI25_HUMAN (24-706): 9.41e-99: 319: 20.96
+	Hs
		1333889 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI45_HUMAN (29-379): 2.85e-68: 237: 20.59
+	Hs
		1318358 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRIM8_HUMAN (16-417): 5.14e-61: 216: 20.34
+	Hs
		1318738 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRIM2_HUMAN (8-524): 1.29e-57: 210: 17.77
+	Hs
		1317493 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI54_HUMAN (184-400): 2.63e-53: 190: 23.96
+	Hs
		1313765 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI62_HUMAN (114-458): 1.79e-32: 133: 15.47
+	Hs
		1336390 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI39_HUMAN (21-389): 6.9e-27: 117: 12.81
+	Hs
		537142 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI27_HUMAN (16-340): 1.18e-23: 107: 14.15
+	Hs
		849366 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI22_HUMAN (23-287): 2.15e-24: 109: 16.48
+	Hs
		584907 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RO52_HUMAN (16-316): 3.12e-24: 108: 13.86
+	Hs
		536760 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI27_HUMAN (16-340): 1.18e-23: 107: 14.15
+	Hs
		135045 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI38_HUMAN (16-269): 6.94e-21: 98.2: 17.12
+	Hs
		135012 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI38_HUMAN (16-269): 6.94e-21: 98.2: 17.12
+	Hs
		734819 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI23_HUMAN (28-272): 6.41e-17: 86.7: 24.71
+	Hs
		734563 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI23_HUMAN (28-272): 6.41e-17: 86.7: 24.71