HitPredict

Interactor 1: KS6A3 (P51812)
Ribosomal protein S6 kinase alpha-3 (S6K-alpha-3) (EC 2.7.11.1) (90 kDa ribosomal protein S6 kinase 3) (p90-RSK 3) (p90RSK3) (Insulin-stimulated protein kinase 1) (ISPK-1) (MAP kinase-activated protein kinase 1b) (MAPK-activated protein kinase 1b) (MAPKAP kinase 1b) (MAPKAPK-1b) (Ribosomal S6 kinase 2) (RSK-2) (pp90RSK2)
Gene: RPS6KA3

Interactor 2: SPRE2 (Q7Z698)
Sprouty-related, EVH1 domain-containing protein 2 (Spred-2)
Gene: SPRED2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.64	0.60	0.621

Property	Details
Experiments	28514442, 32814053
Identification Methods	affinity chromatography technology, two hybrid array, two hybrid pooling approach, anti tag coimmunoprecipitation, validated two hybrid
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0019901 - protein kinase binding
Homologous interactions	7

Homologous interactions

Color coding for hit scores

	1 740 Hs			1 418
+	Hs
		1191416 KS6A6_HUMAN (1-740): 0: 771: 76.54		SPRE2_HUMAN (1-418): 1.5e-175: 498: 100.00
+	Hs
		840477 KS6A1_HUMAN (1-740): 0: 783: 77.90		VASP_HUMAN (9-304): 1.74e-55: 189: 15.64
+	Hs
		1073451 KS6A1_HUMAN (1-740): 0: 783: 77.90		ENAH_HUMAN (9-150): 1.27e-50: 181: 28.08
+	Hs
		1071654 KS6A2_HUMAN (7-327): 3.91e-100: 326: 24.02		SPRE2_HUMAN (1-418): 1.5e-175: 498: 100.00
+	Rn
		139723 AKT3_RAT (390-739): 1.71e-123: 379: 22.10		EVL_RAT (9-314): 6.96e-61: 205: 18.29
+	Hs
		681745 AKT1_HUMAN (327-739): 2.87e-125: 383: 21.02		VASP_HUMAN (9-304): 1.74e-55: 189: 15.64
+	Dm
		1213362 AKT1_DROME (407-736): 3.28e-122: 380: 23.63		ENA_DROME (9-193): 1.68e-47: 176: 23.44